Related Experiment Video
Updated: Jun 27, 2026

Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
The chromosome-level genome assembly of Malus robusta (Rosales: Rosaceae)
Mingxuan Sun1,2, Haibo Wang3, Lingtong Kong1,2
1Beijing Key Laboratory for Agriculture Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China.
Abstract:
Malus robusta Rehd. is widely used as an apple rootstock owing to its strong cold tolerance and desirable rootstock traits. Here, we report Haplotype-resolved, chromosome-level genome assemblies of the cultivar M. robusta, generated using an integrated strategy combining PacBio HiFi sequencing, Hi-C data, and Ultra-long ONT data. Two phased assemblies were produced, with HapA spanning 628.38 Mb and a contig N50 of 36.35 Mb, and HapB spanning 664.60 Mb with a contig N50 of 39.05 Mb. Repetitive elements accounted for 65.12% and 66.52% of the HapA and HapB genomes, respectively. A total of 49,268 and 51,070 protein-coding genes were predicted in HapA and HapB. Genome-wide synteny analysis revealed differential collinearity between the two haplotypes of M. robusta and the genomes of M. baccata and M. prunifolia, respectively. These Haplotype-resolved genome assemblies provide a comprehensive genomic resource for studies of cold tolerance-related traits, comparative genomics, and apple rootstock improvement.
More Related Videos
Related Concept Videos
Genome Annotation and Assembly
Export of Mitochondrial and Chloroplast Genes
Fruit Development, Structure, and Function
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
Chromosome Structure
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...

