Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
07:47

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

Published on: September 15, 2023

Single-Cell Transcriptomic Analysis Identifies an OLFM4-Associated Gastric Cancer Cell State with

Gong Chen1, Weiping Wei2, Dan Li1

  • 1Department of General, Visceral and Transplantation Surgery, University of Heidelberg, 69120 Heidelberg, Germany.

Biomolecules
|June 26, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osteogenic-like Phenotypic Reprogramming Is Associated with Reduced Malignant Behaviors in Pancreatic Cancer Cells Involving MAPK-ERK Signaling.

International journal of molecular sciences·2026
Same author

Multi-Algorithm Analysis Reveals Pyroptosis-Linked Genes as Pancreatic Cancer Biomarkers.

Cancers·2024
Same author

Preparation and properties of copper-oil-based nanofluids.

Nanoscale research letters·2011
Same author

Ordered gelation of chemically converted graphene for next-generation electroconductive hydrogel films.

Angewandte Chemie (International ed. in English)·2011
Same author

A highly selective and sensitive on-off sensor for silver ions and cysteine by light scattering technique of DNA-functionalized gold nanoparticles.

Chemical communications (Cambridge, England)·2011
Same author

The remarkable enhancement of CO-pretreated CuO-Mn2O3/γ-Al2O3 supported catalyst for the reduction of NO with CO: the formation of surface synergetic oxygen vacancy.

Chemistry (Weinheim an der Bergstrasse, Germany)·2011

Researchers identified a specific gastric cancer subtype (C3) linked to OLFM4, showing altered metabolism and palmitoylation. This subtype

Area of Science:

  • Oncology
  • Molecular Biology
  • Metabolomics

Background:

  • Gastric adenocarcinoma (STAD) is characterized by significant intratumoral heterogeneity, impacting tumor progression and treatment response.
  • Understanding the molecular subtypes of STAD is crucial for developing targeted therapies.

Purpose of the Study:

  • To characterize malignant epithelial subtypes in STAD using integrated transcriptomic analyses.
  • To investigate the role of OLFM4 in metabolic regulation within a specific STAD subtype.

Main Methods:

  • Integrated single-cell RNA sequencing and bulk transcriptomic analyses to identify STAD subtypes.
  • Functional enrichment analyses to determine pathway involvement.
  • In vitro experiments with recombinant OLFM4 and siRNA-mediated knockdown in gastric cancer cell lines.
Keywords:
OLFM4gastric cancermetabolic activitiespalmitoylation-related signaturessingle-cell RNA sequencingtumor heterogeneity

More Related Videos

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Related Experiment Videos

Last Updated: Jun 27, 2026

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
07:47

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

Published on: September 15, 2023

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
07:50

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

  • Development of a prognostic risk model using C3 subtype-associated genes.

    • Main Results:

    • Seven malignant epithelial subtypes were identified in STAD, with the OLFM4-associated C3 subtype showing enriched palmitoylation and altered glycolysis.
    • OLFM4 upregulation enhanced glucose uptake and ATP production by increasing ZDHHC2 and GLUT1 expression.
    • OLFM4 silencing decreased ZDHHC2 and GLUT1 expression.
    • A prognostic risk model based on MUC16, RALA, and PCBD1 stratified STAD patients and correlated with immune markers.

    Conclusions:

    • An OLFM4-associated gastric cancer cell state with distinct metabolic and palmitoylation signatures was identified.
    • This metabolic heterogeneity is relevant to gastric adenocarcinoma progression and therapeutic resistance.
    • The prognostic model offers potential for patient stratification and predicting immune responses in STAD.