Related Experiment Video
Updated: Jun 27, 2026
09:17
Minimally Invasive Muscle Embedding (MIME) - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis
Published on: August 24, 2017
Revitalizing Muscle Repair: Hyaluronan Preserves Mitochondrial Architecture and Promotes Myogenesis Under
Fabio Ferrini1, Giosuè Annibalini1, Michela Battistelli1
1Department of Biomolecular Sciences, University of Urbino Carlo Bo, 61029 Urbino, Italy.
Biomolecules
|June 26, 2026
Summary
Hyaluronic acid (HA) mixtures protect muscle stem cells from inflammation, enhancing skeletal muscle repair. This glycomics approach supports muscle regeneration by preserving cell structure and function.
Area of Science:
- Biochemistry
- Cell Biology
- Regenerative Medicine
Background:
- Hyaluronic acid (HA) is a key glycome component crucial for stem cell function and skeletal muscle repair.
- Inflammation can impair muscle regeneration, necessitating strategies to protect myogenic cells.
Purpose of the Study:
- To investigate the effects of a mixture of HA fractions (M-HA) on the repair capacity and myogenic potential of C2C12 myoblasts under inflammatory conditions.
- To assess M-HA's impact on cellular damage, differentiation markers, and ultrastructure.
Main Methods:
- C2C12 murine myoblasts were treated with M-HA (1 mg/mL) under physiological or inflammatory (LPS, IL-1β) conditions.
- Assessed cell viability, scratch wound closure, myogenic index, gene expression (myogenic markers, autophagy), and performed ultrastructural analysis.
Main Results:
- M-HA showed no cytotoxicity and significantly enhanced scratch wound closure.
- M-HA improved the myogenic index, upregulated myogenic markers, preserved myosin heavy chain (MHC) levels, and reduced autophagy-related genes under inflammation.
- Ultrastructural analysis revealed M-HA protected mitochondria and myofibrillar structure from inflammation-induced damage.
Conclusions:
- M-HA protects myoblasts from inflammation-induced damage, supporting skeletal muscle regeneration.
- Glycomics-based strategies using M-HA show potential for enhancing muscle repair in inflammatory environments.
Related Concept Videos
01:21Satellite Stem Cells and Muscular Dystrophy
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
01:23Tissue Renewal without Stem Cells
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
01:13Formation of Muscle Fibers from Myoblasts
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...