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Related Concept Videos

Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Mitochondria01:37

Mitochondria

Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
Mitochondrial Membranes01:45

Mitochondrial Membranes

A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...

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Related Experiment Video

Updated: Jun 27, 2026

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
06:53

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Published on: November 23, 2011

Curcumin Protects SDH2 Mutant from Oxidative Stress and Improves Mitochondrial Function: Application Potential for

Yi Liu1, Na Wang1, Heng Cai1

  • 1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.

International Journal of Molecular Sciences
|June 26, 2026
PubMed
Summary

Curcumin shows promise for treating Complex II deficiency, a rare inherited mitochondrial disorder. This study found curcumin can restore cell viability and mitochondrial function in a yeast model, offering hope for new therapeutic strategies.

Keywords:
complex II deficiencycurcuminmitochondrial disordersoxidative stress

Related Experiment Videos

Last Updated: Jun 27, 2026

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry
06:53

Visualization of Mitochondrial Respiratory Function using Cytochrome C Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

Published on: November 23, 2011

Area of Science:

  • Mitochondrial Biology
  • Biochemistry
  • Pharmacology

Background:

  • Complex II deficiency is a rare inherited mitochondrial disorder with no current drug treatments.
  • Curcumin, a polyphenol, possesses antioxidant and anti-inflammatory properties.
  • A yeast model mimicking human Complex II defects was developed using the *Saccharomyces cerevisiae SDH2* gene.

Purpose of the Study:

  • To investigate the ameliorative effects of curcumin on Complex II functional defects.
  • To explore curcumin's potential as an intervention for mitochondrial diseases.

Main Methods:

  • Constructed a *Saccharomyces cerevisiae* mutant strain mimicking Complex II defects.
  • Assessed cell viability using MTT and CFU assays.
  • Evaluated antioxidant capacity (DCFH-DA, enzyme activity) and gene expression (qRT-PCR).

Main Results:

  • Curcumin restored cell growth and viability in the mutant yeast strain.
  • Curcumin effectively scavenged reactive oxygen species (ROS) and positively regulated mitochondrial function.
  • The observed effects were concentration-dependent.

Conclusions:

  • Curcumin demonstrates potential as a preliminary intervention for Complex II deficiency.
  • These findings support curcumin's application in treating mitochondrial diseases.
  • Further research is warranted to explore curcumin's therapeutic efficacy.