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Active Chitosan Films Enriched with Yerba Mate Kombucha Infusion: Formulation and Characterization.

Celeste Cottet1,2, Pamela A Kikot2,3, Matías L Nobile2,4

  • 1Laboratory of Obtention, Modification, Characterization and Evaluation of Materials (LOMCEM), Department of Science and Technology, National University of Quilmes, Bernal B1876BXD, Argentina.

International Journal of Molecular Sciences
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

This study developed sustainable chitosan films using yerba mate kombucha infusion (YMK-I). The resulting bio-based packaging materials exhibit potent antioxidant and antimicrobial properties, offering a promising alternative to synthetic plastics.

Keywords:
active packagingantioxidant materialsbio-based materialsfood contact materialsnatural plasticization

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Area of Science:

  • Materials Science
  • Food Science
  • Biotechnology

Background:

  • Growing demand for sustainable packaging alternatives to synthetic plastics.
  • Chitosan as a versatile biopolymer for active packaging applications.
  • Yerba mate kombucha infusion (YMK-I) as a source of bioactive compounds.

Purpose of the Study:

  • To develop and characterize chitosan-based films incorporating YMK-I.
  • To evaluate the physicochemical, antioxidant, and antimicrobial properties of these novel films.
  • To assess the potential of YMK-I as a solvent and functional additive in chitosan films.

Main Methods:

  • Preparation of chitosan films using varying concentrations of YMK-I as a solvent.
  • Characterization using rheology, FTIR-ATR, and thermal analyses (DSC/TGA).
  • Assessment of antioxidant activity (radical scavenging) and antimicrobial efficacy against key foodborne pathogens (E. coli, S. aureus).

Main Results:

  • YMK-I demonstrated effective solvent properties for chitosan film formation.
  • Films showed significant antioxidant activity (>85% RSA) and potent antimicrobial effects (>98% inhibition).
  • Incorporation of YMK-I enhanced film flexibility and altered thermal properties.

Conclusions:

  • Chitosan-YMK-I films are multifunctional bio-based materials with promising active packaging potential.
  • The developed films offer a sustainable alternative to conventional plastics.
  • These materials show excellent antioxidant and antimicrobial capabilities for food preservation applications.