Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
Modified-Release Drug Delivery Systems: Classification01:23

Modified-Release Drug Delivery Systems: Classification

Modified-release drug delivery systems improve drug efficacy and minimize side effects by controlling the rate and location of drug release. These systems fall into three categories: rate-programmed, stimuli-activated, and site-targeted.Rate-programmed systems release drugs at a predetermined rate, maintaining consistent therapeutic levels and reducing fluctuations that could lead to toxicity or subtherapeutic effects. These systems use polymeric matrices, reservoir-based designs, or osmotic...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Overlooked Formulation Variable: Solvent Reshapes DNA Delivery.

ACS macro letters·2026
Same author

Concentration of DNA at the Cell Surface Dictates Transfection Efficacy: A Hyperbranched Poly(β-Amino Ester) Mediated Strategy for Enhanced Lentivirus Production.

Polymers·2026
Same author

Probability Method of Molecular Size, Polydispersity, and Branch Unit Distribution Function for the Three-Dimensional Polymers: A Continuation of Flory's Work in 1941.

Macromolecules·2026
Same author

Cyclization in Linear Step-Growth Polymerizations.

Macromolecules·2026
Same author

Peroxisome proliferator-activated receptors-mediated diabetic wound healing regulates endothelial cells' mitochondrial function via sonic hedgehog signaling.

Burns & trauma·2025
Same author

Unique Aerofoil-Structured Microfluidics for High Throughput Lipid Nanoparticle Formulation Screening and Scale-up.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same journal

Tissue MicroRNAs in Arrhythmogenic Cardiomyopathy: A Systematic Review of Studies in Human Myocardium and Animal Models with Implications for Post-Mortem Molecular Diagnostics.

Genes·2026
Same journal

Genetic Variants and Dental Caries Susceptibility: An Umbrella Review and Multilevel Meta-Analysis.

Genes·2026
Same journal

Generative AI and Language Models in Human Genetics and Health: From Variant Interpretation to Clinical Decision Support.

Genes·2026
Same journal

Familial White-Sutton Syndrome Caused by a Pathogenic POGZ p.Arg508* Variant: Intrafamilial Variability from Childhood to Adulthood.

Genes·2026
Same journal

Genetic Influence on LDL-Cholesterol Levels: Role of Polygenic Risk Scores and Lp(a) Beyond Monogenic Hypercholesterolemia.

Genes·2026
Same journal

THBS1 as a Key Regulator of Myoblasts: Validation of Its Inhibitory Roles in Skeletal Muscle Development.

Genes·2026
See all related articles
  1. Home
  3. Polymeric Delivery System For Mrna Therapeutics: Design Principles And Recent Advances.
  1. Home
  3. Polymeric Delivery System For Mrna Therapeutics: Design Principles And Recent Advances.

Related Experiment Video

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Polymeric Delivery System for mRNA Therapeutics: Design Principles and Recent Advances.

Sidi Bao1, Irene Rose Reuben1, Josie Ward1

  • 1Charles Institute of Dermatology, School of Medicine, University College Dublin, D04 V1W8 Dublin, Ireland.

Genes
|June 26, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

Polymeric delivery systems offer a versatile alternative to lipid nanoparticles for messenger RNA (mRNA) therapeutics, enhancing stability and enabling targeted delivery for advanced medical treatments.

Keywords:
CARTdendrimerendosomal escapehybrid nanoparticlesmRNA therapeuticspoly(beta-amino ester)polyethyleneiminepolymeric vectorstargeted delivery

More Related Videos

Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

Related Experiment Videos

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA
08:29

Generation of Cationic Nanoliposomes for the Efficient Delivery of In Vitro Transcribed Messenger RNA

Published on: February 1, 2019

Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded Poly(Beta Aminoesters) Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

Area of Science:

  • Biotechnology and Pharmaceutical Sciences
  • Drug Delivery Systems
  • Molecular Therapy

Background:

  • Messenger RNA (mRNA) therapeutics are revolutionizing medicine, including vaccines, cancer immunotherapy, and gene editing.
  • Lipid nanoparticles (LNPs) have facilitated early clinical successes but face limitations in biodistribution, stability, and tolerability.
  • Polymeric delivery systems present a tunable alternative to LNPs for mRNA complexation, protection, and targeted delivery.

Purpose of the Study:

  • To review recent advancements in polymeric delivery systems for mRNA therapeutics.
  • To highlight strategies for improving mRNA delivery system performance.
  • To identify key challenges for the clinical translation of polymeric mRNA delivery systems.

Main Methods:

  • Review of recent progress in various polymeric systems: polyethyleneimine derivatives, poly(β-amino ester)s, poly(amino acid)s, polyesters, dendrimers, charge-altering releasable transporters, and lipid-polymer hybrids.
  • Analysis of strategies including structural modification, stimuli-responsive designs, and high-throughput polymer screening.
  • Examination of approaches to enhance stability, reduce cytotoxicity, and achieve organ/cell-specific delivery.

    • Main Results:

    • Polymeric systems offer tunable properties for precise control over mRNA delivery.
    • Structural modifications and stimuli-responsive designs improve stability and reduce toxicity.
    • Advanced screening methods accelerate the development of effective polymeric carriers.
    • Organ- or cell-specific delivery is achievable with tailored polymeric formulations.

    Conclusions:

    • Polymeric delivery systems are a promising alternative to LNPs for mRNA therapeutics.
    • Strategies exist to overcome limitations in stability, cytotoxicity, and targeting.
    • Further research addressing immunogenicity, biodistribution, and manufacturing scalability is crucial for clinical success.