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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Environmental Pollutant PCB 153 Is Associated with Candidate Alternative Splicing Alterations in Intellectual

Maria Lui1, Aurelio Minuti1, Simone D'Angiolini1

  • 1IRCCS Centro Neurolesi "Bonino-Pulejo", Via Provinciale Palermo, Contrada Casazza, 98124 Messina, Italy.

Genes
|June 26, 2026
PubMed
Summary

Polychlorinated biphenyls (PCBs) alter gene splicing in brain cells, potentially linking PCB 153 exposure to neurodevelopmental issues and intellectual disability through RNA-binding proteins.

Keywords:
RNA-binding proteinsalternative splicing regulationcomputational splicing analysisintellectual disability disorderspolychlorinated biphenyls

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Area of Science:

  • Environmental Science
  • Neuroscience
  • Molecular Biology

Background:

  • Polychlorinated biphenyls (PCBs) are persistent environmental contaminants.
  • PCB 153 is a prevalent congener linked to toxicity and neurological dysfunction.
  • Post-transcriptional regulation by PCB 153 is poorly understood.

Purpose of the Study:

  • Investigate PCB 153's impact on RNA splicing.
  • Identify differentially alternative splicing events (DASEs) and regulatory mechanisms.
  • Explore potential links to neurodevelopmental dysfunction.

Main Methods:

  • Computational RNA-seq splicing analysis of SH-SY5Y cells exposed to PCB 153.
  • Identification of DASEs, retained introns (RI), and premature termination codons (PTCs).
  • RNA-binding protein (RBP) motif enrichment analysis.

Main Results:

  • PCB 153 exposure altered 32 RNA-binding protein (RBP) genes and 90 DASEs.
  • Affected genes overlapped with intellectual disability and neurodevelopmental gene sets.
  • Specific RBP motifs (CELF2, NUMA1, PRPF8, RBM22) were enriched, suggesting regulatory roles.

Conclusions:

  • Identified candidate PCB 153-associated splicing alterations in a neuronal model.
  • Proposed RBP-mediated regulatory mechanisms for PCB 153's effects.
  • Suggests a potential link between PCB 153, altered splicing, and neurodevelopmental dysfunction.