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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Jun 27, 2026

Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging
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Published on: July 9, 2021

Molecular Visualization of α-Proteobacterial RNA Using a Newly Developed Probe in Extracted Samples, Bacterial Cells,

Juan Xia1, Sora Muramatsu2, Isamu Maeda1,2

  • 1Department of Applied Life Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu 183-8509, Japan.

Microorganisms
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new RNA probe to visualize plant growth-promoting purple non-sulfur bacteria (PNSB) in rice roots. This method allows tracking beneficial bacteria without genetic modification, aiding plant-microbe interaction studies.

Keywords:
16S rRNA probein situ hybridizationpurple non-sulfur bacteriarice rootα-Proteobacteria

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Published on: June 21, 2019

Area of Science:

  • Microbiology
  • Plant Science
  • Molecular Biology

Background:

  • Purple non-sulfur bacteria (PNSB) are known for plant growth promotion.
  • Key agriculturally relevant PNSB belong to α-Proteobacteria.
  • Visualizing these bacteria in plants without genetic modification is difficult.

Purpose of the Study:

  • To develop a molecular tool for visualizing α-Proteobacteria in plant tissues.
  • To investigate the localization of Rhodopseudomonas palustris in rice roots.

Main Methods:

  • Developed a DIG-labeled 16S rRNA gene-based RNA probe.
  • Validated probe specificity using Northern blot and in situ hybridization (ISH) on bacterial cells.
  • Applied ISH to rice roots after inoculation with Rhodopseudomonas palustris C2.

Main Results:

  • The RNA probe specifically hybridized to tested α-Proteobacterial strains.
  • ISH confirmed probe specificity on bacterial cells.
  • Probe-positive bacterial structures were detected in rice root epidermis and root hairs.

Conclusions:

  • The developed RNA probe enables molecular visualization of α-Proteobacteria in plant tissues.
  • This method facilitates studying bacterial localization and plant-bacteria interactions in rice roots.
  • Provides a valuable tool for agricultural microbiology research.