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Related Experiment Video

Updated: Jun 27, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

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Multimodal Optical and Ratiometric ATR-FTIR Discrimination of Mixed Aerosol Components Using pH-Responsive

Chinmaya Mutalik¹, Rachel Redmann¹, Sarah Bose¹

¹Center for Airborne Infection & Transmission Science, Tulane University School of Medicine, New Orleans, LA 70112, USA.

Sensors (Basel, Switzerland)

|June 26, 2026

Summary

Methylcellulose-phenol red films offer a low-cost solution for breath aerosol analysis. These substrates enable simultaneous optical pH sensing and label-free vibrational analysis of aerosol components.

Keywords:

ATR-FTIR spectroscopy bioaerosol detection methylcellulose–phenol red films pH-responsive sensing ratiometric sensing

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Area of Science:

Chemical sensing
Spectroscopy
Biomaterials

Background:

Breath aerosol analysis demands cost-effective sensing materials.
Existing methods struggle to capture and analyze diverse biomolecular components in aerosols.
Need for substrates that preserve chemical information after aerosol capture.

Purpose of the Study:

Evaluate methylcellulose-phenol red (MCPR) films as multimodal sensing substrates for breath aerosols.
Assess the films' ability to capture and analyze model bioaerosols (sulfate, BSA, polystyrene) across a pH range.
Investigate the coupling of optical and vibrational sensing capabilities.

Main Methods:

Utilized model bioaerosols (sodium sulfate, BSA, polystyrene latex) at varying pH.
Employed Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy for vibrational analysis.
Used UV-Visible (UV-Vis) spectroscopy for optical pH sensing.
Performed Scanning Electron Microscopy with Energy Dispersive Spectroscopy (SEM-EDS) for component analysis.

Main Results:

ATR-FTIR showed inverse pH-dependent trends for sulfate and protein spectral regions.
A sulfate-to-protein ratio effectively discriminated ionic and proteinaceous aerosol fractions.
UV-Vis spectroscopy confirmed phenol red's optical responsiveness to pH changes.
FTIR ratio metrics correlated linearly with optical responses, indicating coupled sensing.

Conclusions:

MCPR films function as integrated capture-and-sensing substrates.
The films couple optical pH sensing with label-free vibrational analysis.
MCPR films support the development of low-cost, breath-relevant aerosol sensing platforms.