Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Multilevel Proteome Analysis Reveals the Region-specific Components of the Human Milk Fat Globule Membrane in China.

Journal of agricultural and food chemistry·2026

Same author

The cost of efficiency in flexible neural representations.

bioRxiv : the preprint server for biology·2026

Same author

WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway.

Autophagy·2026

Same author

In-Depth Plasma Proteomics Identifies SNCA as a Discriminating Biomarker of PDAC.

Journal of proteome research·2026

Same author

Enzymatic Basis for the Oxidative Branch of Aromatic Amino Acid Fermentation Leading to p-cresol Formation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Morphology of the Larval Antennae and Mouthparts in <i>Conogethes punctiferalis</i> (Guenée) (Lepidoptera: Crambidae) with Special Reference to Sensilla.

Insects·2026

Search research articles

Related Experiment Video

Updated: Jun 28, 2026

Protein Target Prediction and Validation of Small Molecule Compound

Protein Target Prediction and Validation of Small Molecule Compound

Published on: February 23, 2024

Temperature Range Thermal Proteome Profiling for Drug Target Identification: A Practical Guide.

Yi Xue^1,2, Peiyu Chen², Meining Xing²

¹State Key Laboratory of Component-Based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Jinghai district, Tianjin 301617, China.

Journal of Proteome Research

|June 26, 2026

Summary

This summary is machine-generated.

Thermal proteome profiling (TPP) identifies drug targets. This review details thermal range TPP (TPP-TR) evolution, methods, and data analysis, offering strategies for improved drug discovery and mechanism studies.

Keywords:

drug discovery proteomics target protein thermal proteome profiling

More Related Videos

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Published on: August 9, 2024

Related Experiment Videos

Last Updated: Jun 28, 2026

Protein Target Prediction and Validation of Small Molecule Compound

Protein Target Prediction and Validation of Small Molecule Compound

Published on: February 23, 2024

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Utilizing Thermal Shift Assay to Probe Substrate Binding to Selenoprotein O

Published on: August 9, 2024

Area of Science:

Pharmacology
Biochemistry
Proteomics

Background:

Identifying drug targets and off-targets is essential for understanding drug mechanisms.
Thermal proteome profiling (TPP) is a direct, simple, and scalable method for target identification.
TPP can be applied at the live-cell level to reveal pathway regulation.

Purpose of the Study:

To systematically review the evolution of thermal range TPP (TPP-TR).
To discuss the advantages and limitations of TPP-TR sample preparation and data analysis.
To propose strategies for developing and applying innovative TPP-TR methods.

Main Methods:

Review of existing literature on TPP, focusing on TPP-TR.
Analysis of TPP-TR sample preparation techniques.
Evaluation of TPP-TR data analysis methodologies.

Main Results:

TPP-TR has evolved significantly, becoming a widely practiced TPP format.
Current TPP-TR methods have specific advantages and limitations in sample prep and data analysis.
Improvements in TPP-TR can enhance its application in drug discovery.

Conclusions:

TPP-TR is a valuable tool for drug target identification and mechanism elucidation.
Addressing current limitations in TPP-TR sample preparation and data analysis is crucial.
Further innovation in TPP-TR methods will advance drug discovery research.