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Published on: August 25, 2018
A rice calmodulin-like protein OsCML27 participates in drought tolerance by fine-tuning OsRbohB-mediated ROS
Peng-Tao Shi1,2, Ran Zhang3, Xuan-Jie Wu1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China.
Abstract:
Respiratory burst oxidase homolog (RBOH)-based ROS production was widely verified playing vital roles in plant stress responses; however, its inhibitory mechanism remains largely unknown. Here, we demonstrate that a calmodulin-like (CML) protein OsCML27 functions as a flexible brake of OsRbohB-mediated ROS production to regulate rice drought tolerance via an OsMRLK63-OsCML27-OsRbohB module. OsCML27 negatively regulates drought tolerance of rice where the overexpression lines showed a faster water loss rate and a lower survival rate under drought, while the oscml27 mutants exhibited a slower dehydration rate and a slightly lower survival rate as compared to wild type. OsCML27 can interact with and be phosphorylated by a receptor-like kinase OsMRLK63 primarily at Ser11, and the phosphorylation level could be increased by drought. The phosphorylation by OsMRLK63 enhances OsCML27 stability, and OsCML27 inhibits ROS production by interacting with OsRbohB in the Ca2+-dependent manner. All these results reveal that OsCML27 integrates phosphorylation and Ca2+ signals to regulate drought tolerance of rice by fine-tuning OsRbohB-mediated ROS signaling.
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