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Simultaneous determination of multiple organotin compounds in fish oil by GC-MS following method development and
Wang Liyuan1, Xu Zhenying2, Chen Jing1
1Zhejiang Province Center for Disease Control and Prevention, Hangzhou, 310051, China.
Journal of Chromatography. A
|June 26, 2026
Summary
A new gas chromatography-mass spectrometry (GC-MS) method enhances organotin compound (OTC) detection in fish oil. This optimized method improves accuracy and sensitivity for dietary supplement safety, protecting consumers from neurotoxic substances.
Area of Science:
- Analytical Chemistry
- Food Science
- Environmental Science
Background:
- Organotin compounds (OTCs) in fish oil pose contamination risks and health threats.
- Analytical challenges include complex matrices and OTC degradation.
- Accurate detection is crucial for dietary supplement quality and consumer safety.
Purpose of the Study:
- To develop and optimize a sensitive and efficient gas chromatography-mass spectrometry (GC-MS) method for OTC detection in fish oil.
- To address analytical challenges related to matrix complexity and OTC degradation.
- To provide reliable technical support for the quality and safety monitoring of dietary supplements.
Main Methods:
- Cryogenic extraction, sorbent cleanup, and derivatization with sodium tetraethylborate.
- n-Hexane extraction and further purification.
- Gas chromatography-mass spectrometry (GC-MS) with internal standard quantification.
Main Results:
- Optimized extraction using 3% acetic acid-acetonitrile improved efficiency by over 20%.
- A dual-stage cleanup (HCC18 and Florisil) provided optimal delipidation with >80% recovery.
- The method demonstrated good linearity (R² ≥ 0.99), low LOD/LOQ (0.151-1.317 μg/kg), and high spiked recoveries (81.1%-105%).
Conclusions:
- A systematically optimized dual-stage cleanup strategy significantly enhanced GC-MS performance for OTC detection in fish oil.
- The method effectively eliminated lipid interference, improving accuracy and sensitivity.
- Validated method provides a reliable, high-sensitivity, anti-interference solution for the dietary supplement industry, reducing consumer exposure to neurotoxic OTCs.
