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Updated: Jun 28, 2026
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
Published on: January 12, 2021
A Novel HLA Class I Allele, HLA-B*27:310, Identified Using Next-Generation Sequencing
Ghada Abdelghany1, Michael Ponisciak1, Melanie Hagerty1
1Histocompatibility Laboratory Services, American Red Cross, Philadelphia, Pennsylvania, USA.
HLA
|June 26, 2026
Summary
Researchers discovered a new Human Leukocyte Antigen B (HLA-B) allele, HLA-B*27:310. This novel finding was confirmed using two distinct Next-Generation Sequencing techniques, advancing HLA allele identification methods.
Area of Science:
- Immunogenetics
- Molecular Biology
Background:
- The Human Leukocyte Antigen (HLA) system plays a critical role in immune response.
- Accurate HLA allele identification is crucial for transplantation and disease association studies.
- Novel HLA alleles are continually discovered through advancements in sequencing technologies.
Purpose of the Study:
- To report the discovery and characterization of a novel HLA-B allele.
- To validate the identification of this new allele using multiple sequencing approaches.
Main Methods:
- Next-Generation Sequencing (NGS) was employed for high-throughput genetic analysis.
- Two distinct NGS methodologies were utilized to ensure robust allele identification.
- Bioinformatic analysis was performed to confirm the novel sequence variant.
Main Results:
- A novel HLA-B allele, designated HLA-B*27:310, was identified.
- The allele was consistently detected and confirmed across independent sequencing runs and methods.
- Sequence analysis confirmed its unique status within the HLA-B gene family.
Conclusions:
- The discovery of HLA-B*27:310 expands the known repertoire of HLA-B alleles.
- This finding underscores the utility of NGS in uncovering genetic diversity within the HLA locus.
- Accurate characterization of novel alleles like HLA-B*27:310 contributes to a more comprehensive understanding of immune system variability.
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