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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...

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Related Experiment Video

Updated: Jun 28, 2026

Preparation of Peripheral Blood Mononuclear Cell Pellets and Plasma from a Single Blood Draw at Clinical Trial Sites for Biomarker Analysis
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Rapid Centrifugation for Coagulation Testing: A Multianalyzer Validation Study Harmonizing Preanalytical Protocols

Orakan Limpornpugdee1, Jirapa Kaewkhruawan2, Pitchayaporn Riyagoon2

  • 1Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.

International Journal of Laboratory Hematology
|June 26, 2026
PubMed
Summary

Rapid centrifugation of blood samples provides coagulation test results comparable to standard methods. This approach can enhance laboratory efficiency and support total laboratory automation (TLA) integration.

Keywords:
centrifugationcoagulation assayspreanalytictotal lab automation

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Area of Science:

  • Clinical Laboratory Science
  • Hematology
  • Automation in Healthcare

Background:

  • Coagulation and clinical chemistry testing have differing centrifugation requirements, hindering workflow efficiency and total laboratory automation (TLA). Standard centrifugation for coagulation assays is 1500 g for 15 min, while chemistry assays use 2300 g for 5 min.
  • Optimizing centrifugation protocols is crucial for improving laboratory throughput and automation.

Purpose of the Study:

  • To evaluate the impact of a rapid centrifugation protocol (2300 g for 5 min) on coagulation test results, specifically prothrombin time (PT), activated partial thromboplastin time (APTT), and D-dimer.
  • To determine if rapid centrifugation is compatible with coagulation testing and can be integrated into TLA workflows.

Main Methods:

  • Prospective collection of blood samples from volunteers in 1, 2, and 3 mL citrate tubes.
  • Processing samples using either standard (1500 g for 15 min) or rapid (2300 g for 5 min) centrifugation.
  • Performing PT, APTT, and D-dimer assays on Sysmex CN3000 and ACL-TOP 750 analyzers, with assessment of residual platelet counts.

Main Results:

  • Rapid centrifugation yielded significantly higher residual platelet counts (39.2 × 10^9/L) compared to standard centrifugation (18.8 × 10^9/L).
  • Strong correlations (r > 0.9) were observed for PT and APTT across analyzers. D-dimer showed slightly lower correlation (r = 0.830) on the ACL-TOP 750.
  • Mean biases for PT and APTT were <1%, and D-dimer biases were within total allowable error, with residual platelets showing no significant impact on results.

Conclusions:

  • Rapid centrifugation (2300 g for 5 min) produces coagulation results comparable to the standard protocol.
  • This rapid method may facilitate the integration of coagulation testing into total laboratory automation (TLA) workflows, improving efficiency.