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In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
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Rapid and Continuous Directed Evolution in Vibrio natriegens Utilizing an In Vivo Hypermutation System

Ruiying Zhu1,2,3, Zehua Bao1,2,3,4

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang 310058, China.

ACS Synthetic Biology
|June 26, 2026
PubMed
Abstract

Keywords:
T7 RNA polymeraseTEM-1Vibrio natriegensdeaminasedirected evolutiongene diversification

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