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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Genome-wide Association Studies-GWAS01:11

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

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Genomic Confirmation of HLA-B*15:591 by Next Generation Sequencing.

Jarret Merschman1, Derek Benidt1, Justin Kreuter1

  • 1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

HLA
|June 26, 2026
PubMed
Summary

A novel Human Leukocyte Antigen B allele, HLA-B*15:591, has been identified. This allele is distinguished by a specific genetic mutation in exon 4, impacting its structure.

Keywords:
HLA‐B*15:591allele confirmationhistocompatibilityimmunogeneticsnext generation sequencing

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Last Updated: Jun 28, 2026

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08:07

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Published on: January 12, 2021

Area of Science:

  • Immunogenetics
  • Molecular biology

Background:

  • The Human Leukocyte Antigen (HLA) system plays a critical role in immune response.
  • Genetic variations within HLA genes contribute to diverse immune profiles.

Purpose of the Study:

  • To characterize a newly identified HLA-B allele, designated HLA-B*15:591.
  • To describe the specific genetic alteration defining this allele.

Main Methods:

  • Sequence analysis of the HLA-B gene.
  • Identification of nucleotide substitutions.

Main Results:

  • The HLA-B*15:591 allele is defined by a single nucleotide substitution.
  • This substitution occurs at codon 219 within exon 4 of the HLA-B gene.

Conclusions:

  • The characterization of HLA-B*15:591 expands the known diversity of HLA alleles.
  • Understanding such variations is crucial for immunogenetic studies and transplantation research.