Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial: Harnessing the potential of duckweed: biological insights and ecological applications.

Frontiers in plant science·2026
Same author

PET/CT contouring techniques in neuroendocrine neoplasms: a state-of-the-art scoping review.

EJNMMI reports·2026
Same author

Impact of Canadian Association of Radiologists and Fukuoka Recommendations on MRI Utilization for Surveillance of Incidental Pancreatic Cysts.

Canadian Association of Radiologists journal = Journal l'Association canadienne des radiologistes·2026
Same author

Inter and intra-observer agreement for the LST Classification in Large (>2cm) Colorectal Laterally Spreading Tumours.

The American journal of gastroenterology·2026
Same author

Endosonography-guided choledochoduodenostomy using a lumen apposing metal stent vs. ERCP: Individual patient data and aggregate meta-analyses.

Endoscopy international open·2026
Same author

Triploidy is prominent in the duckweed Lemna minor complex.

Communications biology·2026

Related Experiment Video

Updated: Jun 28, 2026

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing
05:58

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing

Published on: August 16, 2024

Single-cell RNA sequencing in thyroid cancer: a methodological review and thyroid specific dissociation protocol.

Sumathy Perampalam1,2,3, Eric Lam4, Georgia Rankin4

  • 1Endocrinology, The Canberra Hospital, GARRAN, ACT, 2605, Australia. sumathy.perampalam@act.gov.au.

Thyroid Research
|June 26, 2026
PubMed
Summary

This study developed an optimized protocol for single-cell RNA sequencing of thyroid cancer, improving cell recovery and viability. This method enhances the discovery of biomarkers and therapeutic targets for aggressive thyroid carcinomas.

Keywords:
ScRNA-seqSingle cell sequencingThyroid cancerThyroid tissue dissociation protocolTissue dissociation protocol

More Related Videos

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

Related Experiment Videos

Last Updated: Jun 28, 2026

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing
05:58

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing

Published on: August 16, 2024

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method
07:00

Nuclei Isolation from Whole Tissue using a Detergent and Enzyme-Free Method

Published on: June 24, 2020

Area of Science:

  • Endocrinology
  • Oncology
  • Genomics

Background:

  • Thyroid cancer is the most common endocrine malignancy, with high-grade subtypes exhibiting aggressive behavior and poor survival.
  • Current treatments like multikinase inhibitors offer limited durability for radioactive iodine-refractory disease.
  • Understanding molecular differences between thyroid cancer subtypes is crucial but hindered by limited high-resolution data.

Purpose of the Study:

  • To critically evaluate existing single-cell RNA sequencing (scRNA-seq) studies in thyroid cancer.
  • To identify methodological gaps in current scRNA-seq applications for thyroid cancer.
  • To develop and present an optimized protocol for generating high-quality single-cell suspensions from thyroid specimens for scRNA-seq.

Main Methods:

  • A focused literature search of PubMed and Embase identified scRNA-seq studies of primary thyroid tumors (2020-2025).
  • Methodological details of tissue dissociation were assessed in 22 selected studies.
  • An optimized thyroid tissue dissociation protocol was developed, replacing RBC lysis with immunomagnetic depletion.

Main Results:

  • Many published studies lacked detailed methods for tissue dissociation.
  • The optimized protocol improved cell recovery and viability compared to standard lysis in limited specimens.
  • The protocol yields high-viability single-cell suspensions compatible with droplet-based scRNA-seq platforms.

Conclusions:

  • The optimized dissociation protocol is practical for precious patient-derived thyroid samples.
  • Improved single-cell data quality and cellular representation facilitate biomarker discovery for disease progression and treatment resistance.
  • This approach supports the identification of novel therapeutic targets across diverse thyroid cancer subtypes.