Non-destructive and accurate phenotypic detection method for okra seedlings under salt stress based on dual-view...

Soil salinization has become a critical factor limiting global agricultural production. Characterizing the growth and developmental responses of okra to salt stress and developing efficient and accurate salt-stress phenotyping techniques can provide an important methodological reference for okra cultivation in saline lands and future multi-cultivar salt-stress phenotyping studies. Traditional manual measurement of plant phenotypic parameters suffers from low efficiency and insufficient detection accuracy, making it difficult to achieve rapid and non-destructive analysis of plant phenotypic traits under salt stress. Therefore, this study proposes a computational phenotyping parameter extraction method based on the CSP-MSG Net model. Using dual-view feature fusion, we constructed a dedicated dataset. On the basis of PointNet++-MSG, the original MLP layers were replaced with C2F modules, and the SGE attention mechanism was integrated to enhance morphological feature extraction, thereby constructing a lightweight CSP-MSG Net architecture adapted to okra seedling point clouds for semantic segmentation of okra point clouds combined with DBSCAN clustering to complete instance segmentation, phenotypic parameters including plant height, stem diameter and canopy width were further calculated. This scheme enables high-throughput data acquisition, improves measurement accuracy, effectively reduces model parameters and computational overhead, and realizes lightweight operational performance. The results show that okra seedlings can still grow with increasing salt stress concentration, while the growth rates of the three measured traits are all inhibited, indicating that high-concentration salt stress impairs the growth activity of okra seedlings. To verify the calculation accuracy of the model, the phenotypic parameters predicted by the model were compared with manually measured values. The coefficients of determination for stem diameter, canopy width and plant height of okra seedlings reached 0.96, 0.99 and 0.99, respectively. These results strongly demonstrate the excellent reliability and effectiveness of the proposed method, providing methodological support for non-destructive and accurate phenotypic detection of okra seedlings under salt stress.