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Published on: August 23, 2019
Valorization of wine industry by-products as a flavoring agent in water kefir: microbiological viability and
Ana Carolina Mendes Oliveira Campos1, Ana Elisa Barbosa Siqueira2, Maria Isabela da Silva Figueiredo3
1Food and Nutrition Department, Faculty of Nutrition, Federal University of Mato Grosso (UFMT), Cuiabá, Brazil.
Introduction:
This study developed a functional water kefir beverage flavored with grape pomace (Vitis labrusca.) and evaluated its physicochemical, microbiological, and sensory properties, alongside its functional effects in a Caenorhabditis elegans model.
Methods:
Four treatments were tested: 0% (KC), 25% (K25), 37.5% (K37.5), and 50% (K50) pomace addition.
Results:
Anthocyanin and phenolic contents were dose-dependent, peaking in K50 (33.56 mg L-1 and 4.31 mg g-1, respectively). During 21 days of refrigerated storage, a decrease in reducing sugars, pH (4.3 to 3.5), and a concomitant increase in acidity (up to 6.60%) were observed, reflecting continuous microbial metabolic activity. Due to excessive acidity and residual taste, K37.5 and K50 were deemed unfeasible. Consequently, K25 was selected for biological and sensory validation. In K25, yeast and acetic acid bacteria (AAB) maintained high viability (107 CFU mL-1), whereas lactic acid bacteria (LAB) remained below 105 CFU mL-1 due to the selective pressure of the grape pomace. K25 achieved a sensory acceptance index of 76.11% and a 65% purchase intention.
Discussion:
These results indicate that water kefir flavored with 25% grape pomace is a viable probiotic carrier rich in bioactive compounds with promising sensory appeal.
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