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ENTPD2 Transcript-Protein Divergence in Colorectal Cancer and Its Association with miR-708-5p: An Integrative

Leyla Ataç Doğan¹

¹Department of Medical Biology, Tayfur Sökmen Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Türkiye.

Omics : a Journal of Integrative Biology

|June 29, 2026

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Ectonucleoside triphosphate diphosphohydrolase 2 (ENTPD2) mRNA is elevated in colorectal cancer (CRC), but protein levels are lower. MicroRNA-708-5p targets ENTPD2, suggesting a regulatory role in CRC progression.

Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Ectonucleoside triphosphate diphosphohydrolase 2 (ENTPD2) is involved in nucleotide metabolism and purinergic signaling.
Its role in colorectal cancer (CRC) and tumor-immune interactions is not well understood.

Purpose of the Study:

To investigate the expression pattern and regulatory mechanisms of ENTPD2 in colorectal cancer.
To explore the potential role of microRNAs in regulating ENTPD2 in CRC.

Main Methods:

Integrative analysis of transcriptomic, proteomic, miRNA, and single-cell transcriptomic datasets.
Bioinformatic analysis to identify potential miRNA regulators.
Functional validation using dual-luciferase reporter assays, qRT-PCR, and Western blot in CRC cell lines.

Keywords:

ENTPD2 colorectal cancer miR-708-5p post-transcriptional control

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Main Results:

ENTPD2 mRNA levels were elevated in CRC tissues, correlating with shorter relapse-free survival.
Protein levels of ENTPD2 were reduced in CRC samples, indicating transcript-protein divergence.
miR-708-5p was identified as a potential regulator, with increased expression in CRC.
miR-708-5p was experimentally validated to directly target ENTPD2 3'-UTR and suppress its expression in CRC cells.

Conclusions:

ENTPD2 expression is dysregulated in colorectal cancer at both mRNA and protein levels.
miR-708-5p plays a significant role in post-transcriptional regulation of ENTPD2 in CRC.
These findings provide insights into the molecular mechanisms underlying ENTPD2 regulation in CRC.