Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same journal

Integrative Biological Analysis of Histamine Depletion on Neuroinflammation, Dopamine D2 Receptor, and Glutamate in Experimental Tic Disorder.

Omics : a journal of integrative biology·2026

Same journal

Multiomics Integration Reveals Distinct miRNA-Mediated Regulatory Networks at the Transcript and Protein Levels in Idiopathic Pulmonary Fibrosis.

Omics : a journal of integrative biology·2026

Same journal

Synergistic Geroprotectors Mapping through Systems Machine Learning and Graph Neural Networks.

Omics : a journal of integrative biology·2026

Same journal

<i>ENTPD2</i> Transcript-Protein Divergence in Colorectal Cancer and Its Association with miR-708-5p: An Integrative Analysis.

Omics : a journal of integrative biology·2026

Same journal

Integrated Bioinformatics and Molecular Docking Analysis Reveals Potential Mechanisms of Coptidis Rhizoma in Intervertebral Disc Degeneration.

Omics : a journal of integrative biology·2026

Same journal

Site-Specific Phosphoproteomics Uncovers Potential Regulatory Networks of NEK4 in DNA Damage Response and Cancer Progression.

Omics : a journal of integrative biology·2026

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Characterizing Extracellular Vesicles from Biological Fluids

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

Human Extracellular Vesicles, Metabolites, and Metabolomics: A Comprehensive Review.

Mohammed A Al-Zubaidi¹

¹Department of Clinical Laboratory Sciences, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq.

Omics : a Journal of Integrative Biology

|July 14, 2026

Summary

Extracellular vesicle (EV) metabolomics offers insights into cell communication and disease. Challenges in EV isolation and analysis are being addressed by advanced techniques and multi-omics integration for clinical applications.

Keywords:

biology systems biomarkers extracellular vesicles extracellular vesicles challenges metabolomics

More Related Videos

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jul 15, 2026

Characterizing Extracellular Vesicles from Biological Fluids

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Single Extracellular Vesicle Transmembrane Protein Characterization by Nano-Flow Cytometry

Published on: July 26, 2022

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

Biochemistry
Cell Biology
Systems Biology

Background:

Metabolomics provides a systems-level view of biochemical pathways and metabolic phenotypes.
Extracellular vesicles (EVs) are key mediators of intercellular communication, carrying metabolites reflective of their parent cell.
EVs are rich in lipids, proteins, nucleic acids, and metabolites involved in signaling and disease pathogenesis.

Purpose of the Study:

To critically synthesize current knowledge in EV metabolomics.
To address methodological and analytical challenges in EV metabolomics research.
To explore emerging clinical applications and future research directions.

Main Methods:

Review of analytical technologies for EV metabolomics.
Examination of statistical and computational approaches.
Discussion of multi-omics integration and machine learning strategies.

Main Results:

EV metabolomics is methodologically and analytically challenging due to vesicle heterogeneity, isolation purity, and detection sensitivity issues.
Methodological variability, contamination, and study design limitations impact reproducibility and translation.
Multi-omics integration and machine learning show promise for biomarker discovery and biological system interpretation.

Conclusions:

Advancements in analytical technology and computational approaches are crucial for overcoming EV metabolomics challenges.
Addressing methodological variability and improving study design are essential for reproducible and translatable research.
Future research should focus on multi-omics integration and machine learning to advance clinical applications in translational medicine.