Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic Profiling of Oxidative Stress Response Proteins with a Methionine Sulfoxide-Inspired Activity-Based Probe.

JACS Au·2026
Same author

Intracristal space proteome mapping using super-resolution proximity labeling with isotope-coded probes.

Nature communications·2025
Same author

Chemoproteomic identification of phosphohistidine acceptors: posttranslational activity regulation of a key glycolytic enzyme.

Chemical science·2025
Same author

Bringing Histidine Phosphorylation into Light: Role of Chemical Tools.

ACS chemical biology·2025
Same author

Identification of a Target Site for Covalent Inhibition of Protein Phosphohistidine Phosphatase 1.

ACS medicinal chemistry letters·2022
Same author

Ir and NHC Dual Chiral Synergetic Catalysis: Mechanism and Stereoselectivity in γ-Butyrolactone Formation.

Journal of the American Chemical Society·2022
Same journal

Multimodal Detection of Low Water Contents in Ethanol Using a Plasmon-Berreman-Enhanced Metasurface Infrared Absorber.

ACS sensors·2026
Same journal

3D-Printed Hollow Microneedle Potentiometric Sensors: A Modular Approach.

ACS sensors·2026
Same journal

Single-Atom Ni-Modified SnO<sub>2</sub> for Ultrasensitive NO<sub>2</sub> Gas Sensing through Enhanced Molecular Adsorption and Efficient Charge Transfer.

ACS sensors·2026
Same journal

Harnessing Thermoelectric-Mediated Photoelectrochemical System to Address Sensitive Dopamine Detection via APE1-Amplified Triple-Helix Switching.

ACS sensors·2026
Same journal

Ultrasensitive Detection of Mold Biomarker 1-Octen-3-ol Using AuPt Nanocluster-Sensitized WO<sub>3</sub> Gas Sensor for On-Site Grain Safety Monitoring.

ACS sensors·2026
Same journal

Bottom-Up Absorptive and Stretchable Plasmonic Tape for Field-Deployable In Vivo Fruit Safety Surveillance.

ACS sensors·2026
See all related articles

Related Experiment Video

Updated: Jul 1, 2026

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
07:45

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Published on: June 6, 2022

A Genetically Encoded Fluorescent Sensor for Protein Arginine Phosphorylation.

Hoyoung Jung1, Shin Hyeon Lee1, Jung-Min Kee1

  • 1Department of Chemistry, Ulsan National Institute of Science and Technology (UNIST), Ulsan Metropolitan City 44919, Republic of Korea.

ACS Sensors
|June 29, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed FLAP, a fluorescent sensor for real-time monitoring of protein arginine phosphorylation (pArg). This breakthrough enables studying pArg dynamics and related enzymes in live cells, crucial for understanding bacterial protein degradation and developing new therapeutics.

Keywords:
arginine kinasearginine phosphorylationfluorescent sensorgenetically encoded sensorlive-cell microscopyphosphoarginine phosphatase

More Related Videos

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
08:21

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

Related Experiment Videos

Last Updated: Jul 1, 2026

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
07:45

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation

Published on: June 6, 2022

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor
08:21

Real-time In Vivo Recording of Arabidopsis Calcium Signals During Insect Feeding Using a Fluorescent Biosensor

Published on: August 15, 2017

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Microbiology

Background:

  • Protein arginine phosphorylation (pArg) is a critical post-translational modification (PTM) in bacteria, acting as a degron for protein degradation.
  • Enzymes regulating pArg are potential therapeutic targets against drug-resistant bacteria.
  • Current methods for monitoring pArg are limited, especially for live-cell applications, due to pArg's chemical instability.

Purpose of the Study:

  • To develop a novel method for real-time monitoring of pArg dynamics.
  • To create a genetically encoded fluorescent sensor for pArg.
  • To investigate the activity of arginine kinase McsB and pArg phosphatase YwlE.

Main Methods:

  • Development of FLAP (FLuorescent Arg Phosphorylation sensor), a genetically encoded FRET-based sensor.
  • In vitro characterization of FLAP's response to phosphorylation and dephosphorylation.
  • Testing FLAP in an orthogonal E. coli system to detect McsB-dependent pArg formation.

Main Results:

  • FLAP demonstrated reversible, real-time FRET changes correlating with arginine phosphorylation and dephosphorylation in vitro.
  • FLAP successfully detected McsB-mediated pArg formation in E. coli upon McsB expression.
  • The sensor provides a proof of concept for live-cell detection of Arg phosphorylation.

Conclusions:

  • FLAP is a novel genetically encoded platform for studying pArg-writing and pArg-erasing enzymes.
  • This sensor overcomes limitations in monitoring pArg dynamics, enabling in vitro and potentially live-cell studies.
  • FLAP facilitates research into bacterial protein degradation pathways and the development of novel antibacterial strategies.