Ebselen-loaded silver-containing mesoporous bioactive glass for the control of Enterococcus faecalis and...

Area of Science:

• Biomaterials Science
• Endodontics
• Nanotechnology

Background:

• Calcium silicate-based sealers have limitations against persistent endodontic biofilms.
• Developing advanced bioceramics is crucial for improving endodontic treatment outcomes.

Purpose of the Study:

• To synthesize and characterize a novel bioceramic material, Ebselen/MBG-Ag.
• To evaluate its antibacterial, antibiofilm, hydroxyapatite (HA)-formation, and cytocompatibility properties for endodontic applications.

Main Methods:

• Mesoporous bioactive glass loaded with silver (MBG-Ag) was synthesized and further loaded with Ebselen.
• Characterization included FTIR, TEM, and EDS.
• Antibacterial, antibiofilm, HA-formation, and cytotoxicity assays were performed against key endodontic pathogens.

Main Results:

• Ebselen/MBG-Ag demonstrated sustained release of silver ions and Ebselen.
• Significantly enhanced antibacterial and antibiofilm activities against Enterococcus faecalis and Streptococcus mutans were observed.
• Rapid HA formation on dentin and improved in vitro cytocompatibility were noted.

Conclusions:

• Ebselen/MBG-Ag exhibits superior antibacterial and antibiofilm efficacy compared to MBG-Ag alone.
• The material promotes dentin remineralization and shows reduced cytotoxicity.
• Ebselen/MBG-Ag is a promising candidate for next-generation endodontic sealers.