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Enhancing the conception rate in water buffalo using post-AI intravaginal bio-stimulation and clitoral massage
Anup Sarker1, Dibyendu Biswas1, Ashit Kumar Paul1
1Department of Medicine, Surgery and Obstetrics, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal, Bangladesh.
Open Veterinary Journal
|June 30, 2026
Summary
Improving buffalo conception rates in Bangladesh requires enhanced artificial insemination (AI) techniques. Combining intravaginal bio-stimulation with clitoral massage (CM) significantly boosts post-AI success in buffalo farming.
Area of Science:
- Veterinary Science
- Animal Reproduction
Background:
- Suboptimal reproductive performance after artificial insemination (AI) impacts buffalo farming profitability in Bangladesh.
- Existing AI protocols may not fully optimize conception rates in buffaloes.
Purpose of the Study:
- To evaluate the efficacy of intravaginal bio-stimulation and clitoral massage (CM) in improving post-AI conception rates in buffaloes.
- To compare different AI enhancement strategies including penis-like device (PLD) and CM.
Main Methods:
- 125 female buffaloes were divided into five groups: natural mating, AI during estrus, AI with PLD, AI with CM, and AI with PLD and CM.
- Conception rates were recorded and analyzed, with factors like age, parity, and reproductive health considered.
Main Results:
- The combined PLD and CM group (Group E) showed a 52.0% conception rate, higher than AI alone (32.0%).
- Parity, previous calving difficulty, and age (4.6-5.5 years) significantly influenced conception rates (p < 0.05).
- A positive correlation was found between age and parity, and a negative correlation with reproductive health.
Conclusions:
- Intravaginal bio-stimulation using a PLD combined with CM post-AI significantly improves conception rates in buffaloes.
- These findings offer a practical approach to enhance buffalo reproductive efficiency and farm profitability.
