Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Genetic Screens02:46

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...
Mutagenicity and Carcinogenicity01:25

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pannexin1-dependent and -independent protection by novel peptidomimetics against cardiac ischemia/reperfusion injury.

Scientific reports·2026
Same author

A combined in silico and in vitro new approach methodology for early detection of liver steatogenic chemicals.

Chemico-biological interactions·2026
Same author

QSAR in the AI Era: Reflections for Advancing Chemical Safety Assessment.

Chemical research in toxicology·2026
Same author

To What Extent Do Fish Toxicity Studies Drive Harmonised Classification for Acute and Chronic Aquatic Hazards?

Environmental toxicology and chemistry·2026
Same author

Stakeholder input towards further refinement and consolidation of the alternative safety profiling algorithm (ASPA) for next generation risk assessment (NGRA).

ALTEX·2026
Same author

Lost in NAMs-lation: A review of animal-free science definitions.

ALTEX·2026

Related Experiment Video

Updated: Jul 1, 2026

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

GenoITS: Implementation of an Integrated Testing Strategy workflow for genotoxicity using QSAR-based tools.

José Luis Vallés-Pardo1, Eva Serrano-Candelas1, Addel Goya-Jorge1

  • 1ProtoQSAR S.L., Paterna, (València), Spain.

NAM Journal
|June 30, 2026
PubMed
Summary

This study introduces GenoITS, a computational approach to assess chemical genotoxicity, reducing animal testing and costs. The workflow integrates various assays and QSAR models for regulatory compliance.

Keywords:
GENOTOXICITYITSNAMsQSAR

More Related Videos

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox
05:47

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox

Published on: August 28, 2019

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
10:27

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

Related Experiment Videos

Last Updated: Jul 1, 2026

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox
05:47

In Silico Modeling Method for Computational Aquatic Toxicology of Endocrine Disruptors: A Software-Based Approach Using QSAR Toolbox

Published on: August 28, 2019

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts
10:27

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

Area of Science:

  • Computational toxicology
  • Regulatory science
  • Chemical safety assessment

Background:

  • EU REACH regulation mandates extensive chemical testing, posing economic and ethical challenges.
  • Standard Information Requirements include genotoxicity assays, often involving animal testing.
  • Minimizing animal use (3Rs) is a key regulatory objective.

Purpose of the Study:

  • To develop an exclusively computational Integrated Testing Strategy for chemical genotoxicity assessment.
  • To create an automated workflow, GenoITS, to streamline regulatory testing.
  • To reduce the economic burden and ethical concerns associated with traditional genotoxicity testing.

Main Methods:

  • Integration of diverse genotoxicity assays (gene mutation, cytogenotoxicity) into an automated workflow.
  • Utilization of in-house Quantitative Structure-Activity Relationship (QSAR) models to fill data gaps.
  • Development of GenoITS as part of the ProtoPRED prediction suite, following REACH genotoxicity assessment schema.

Main Results:

  • GenoITS provides an automated workflow for genotoxicity testing.
  • The system combines assay data and QSAR predictions for a binary classification (genotoxic/not genotoxic).
  • The workflow is designed to meet regulatory requirements for chemical safety assessment.

Conclusions:

  • The GenoITS workflow offers a viable computational alternative for genotoxicity testing.
  • This approach supports regulatory compliance while adhering to the 3Rs principles.
  • The web-based platform benefits the scientific community navigating REACH and other chemical regulations.