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Updated: Jul 1, 2026
08:30
One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
Published on: July 9, 2012
Rapid Detection of Pathogens and Resistance Genes in Positive Blood Cultures Using BioFire® FilmArray BCID2
Canberk Çinar1, Yeliz Tanriverdi Çayci1, Zehranur Atakcan1
11Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayıs University, Samsun, Turkey.
Polish Journal of Microbiology
|June 30, 2026
Summary
The BioFire FilmArray Blood Culture Identification 2 (BCID2) panel rapidly and accurately identifies bloodstream pathogens and antimicrobial resistance genes. This molecular test complements traditional methods for timely patient treatment.
Area of Science:
- Clinical Microbiology
- Molecular Diagnostics
- Infectious Diseases
Background:
- Bloodstream infections (BSIs) are a major cause of illness and death, necessitating swift pathogen identification and antimicrobial resistance (AMR) profiling for effective treatment.
- Conventional culture-based methods for diagnosing BSIs are time-consuming, delaying critical therapeutic decisions.
- Molecular diagnostic systems offer a faster alternative for pathogen detection and AMR gene identification.
Purpose of the Study:
- To evaluate the performance of the BioFire FilmArray Blood Culture Identification 2 (BCID2) panel for the rapid detection of microorganisms and AMR genes directly from positive blood cultures.
- To compare the results obtained from the BCID2 panel with conventional culture-based methods, mass spectrometry, and automated antimicrobial susceptibility testing.
Main Methods:
- Prospective evaluation of 50 positive peripheral blood cultures using the BCID2 panel.
- Parallel analysis with conventional culture, mass spectrometry identification, and automated antimicrobial susceptibility testing.
- Confirmation of detected resistance genes using polymerase chain reaction (PCR).
Main Results:
- The BCID2 panel detected at least one microorganism in 94% of samples, demonstrating strong agreement with culture-based methods.
- High concordance was observed for *Escherichia coli* and *Pseudomonas aeruginosa*; *Klebsiella pneumoniae* showed acceptable agreement.
- Polymicrobial infections were identified in 40% of cases, and detected resistance genes correlated well with phenotypic resistance.
Conclusions:
- The BCID2 panel provides rapid and reliable identification of common bloodstream pathogens and key antimicrobial resistance genes.
- This molecular system serves as a valuable adjunct to conventional diagnostics, enhancing the speed of BSI management.
- Interpretation of results, especially for less common organisms, benefits from integration with clinical data and repeat blood culture information.
Keywords:
BioFire FilmArray Blood Culture Identification 2antimicrobial resistanceblood culturebloodstream infectionsmolecular diagnosticsMore Related Videos
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