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Updated: Jul 2, 2026
05:38
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
Exploring molecular characterization circulating influenza A and B viruses in Tehran, Iran
Ali Maleki1,2, Mohammad Hadi Karbalaei Niya3, Mona Roozbehani4
1Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Pasteur Ave, Tehran, Iran.
Summary
This study tracked influenza A and B virus evolution in Tehran, identifying H3N2 and H1N1pdm09 subtypes and B/Yamagata lineage. Findings aid in understanding viral changes for public health preparedness.
Area of Science:
- Virology
- Epidemiology
- Molecular Biology
Background:
- Influenza A and B viruses cause seasonal flu outbreaks, necessitating pandemic control strategies.
- Understanding regional virus circulation is crucial for effective public health interventions.
Purpose of the Study:
- To identify the molecular and seasonal patterns of influenza virus subtypes.
- To investigate circulating influenza A and B viruses in Tehran, Iran.
Main Methods:
- Cross-sectional study of 108 positive clinical samples from 2023-2024.
- Conventional PCR and sequencing of HA and NA gene fragments for mutation analysis.
- Phylogenetic analysis of influenza virus isolates.
Main Results:
- Influenza A viruses (IAV) accounted for 64.81% (70/108) and Influenza B viruses (IBV) for 35.18% (38/108).
- Dominant IAV subtypes were H3N2 (64.28%) and H1N1pdm09 (35.71%).
- Influenza B cases were characterized as B/Yamagata lineage, with detected amino acid substitutions in HA1 subunits of H1N1 and H3N2.
Conclusions:
- Phylogenetic and sequencing data provide essential information for tracking influenza virus evolution.
- Findings support vaccine development and enhance public health preparedness against influenza.
- Continuous monitoring of viral evolution is vital for global health initiatives.
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