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Updated: Jul 4, 2026
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Isolation of Nuclei from Human Intermuscular Adipose Tissue and Downstream Single-Nuclei RNA Sequencing
Published on: May 3, 2024
Transcriptome and co-expression network analysis identified candidate genes for intramuscular fat deposition in
H Wang1,2, C Wang1,2, W Zhao1,2
1College of Animal Science and Technology, Ningxia University, Yinchuan, China.
British Poultry Science
|July 3, 2026
Summary
This study reveals key genes regulating intramuscular fat deposition in Jingyuan chickens across development. Findings offer insights into molecular breeding for enhanced meat quality.
Area of Science:
- Animal Science
- Genomics
- Molecular Biology
Background:
- Intramuscular fat (IMF) deposition is crucial for meat quality.
- Understanding the genetic regulation of IMF deposition is vital for poultry breeding.
- Jingyuan chickens are a breed of interest for meat production.
Purpose of the Study:
- To investigate the regulation of intramuscular fat deposition in Jingyuan chickens at different developmental stages.
- To identify candidate genes and molecular pathways involved in IMF deposition.
- To provide a transcriptomic resource for improving meat quality through molecular breeding.
Main Methods:
- Transcriptome sequencing of pectoral muscles from Jingyuan hens at 42, 126, and 180 days of age.
- Weighted Gene Co-expression Network Analysis (WGCNA) to identify gene modules correlated with IMF and age.
- Differential Gene Expression (DEG) analysis to pinpoint key genes.
Main Results:
- Identified 1187, 1377, and 617 differentially expressed genes across developmental stages.
- Discovered significant gene modules (MEyellow, MEbrown, MEdarkred) associated with IMF content and age.
- Highlighted candidate genes like MAPK8, ACSL4, ACACB, FOXO6, PTEN, EGFR, AGXT2, and LAMB1 involved in fat metabolism and signaling pathways.
Conclusions:
- Identified key genes and pathways regulating intramuscular fat deposition in Jingyuan chickens.
- Candidate genes AGXT2 and LAMB1 show potential for influencing IMF deposition.
- This transcriptomic data serves as a valuable resource for molecular breeding strategies to enhance chicken meat quality.
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