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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Updated: Jul 4, 2026

Recapitulation of an Ion Channel IV Curve Using Frequency Components
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Published on: February 8, 2011

Quantification of Human Cardiac Ion Channels by Parallel Reaction Monitoring and In-Sample Calibration.

Soroush Torkamannejad1, Hoi-Ying Yip2, Bingyun Sun1

  • 1Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A1S6, Canada.

ACS Omega
|July 3, 2026
PubMed
Summary
This summary is machine-generated.

This study quantifies cardiac ion channel subunits in the human ventricle using targeted proteomics. The developed method provides absolute copy numbers for key channels like Nav1.5, hERG, and Cav1.2, aiding arrhythmia research.

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Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
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Area of Science:

  • Cardiovascular Biology
  • Proteomics
  • Molecular Cardiology

Background:

  • Cardiac ion channels are crucial for cardiomyocyte action potentials.
  • Their dysfunction leads to electrical remodeling and arrhythmia.
  • Quantifying these large transmembrane proteins is challenging.

Purpose of the Study:

  • To quantify ion channel subunits in the human ventricle using a targeted proteomic approach.
  • To establish a method for determining absolute copy numbers of cardiac ion channels.
  • To improve the detection of large membrane proteins in proteomic studies.

Main Methods:

  • Utilized a targeted parallel reaction monitoring (PRM) proteomic approach.
  • Applied extended enzymatic digestion to sodium deoxycholate (SDC) insoluble fractions.
  • Employed Heavy-Match-Light (HML) in-sample calibration for quantification.

Main Results:

  • Successfully quantified major ion channel subunits: Nav1.5, hERG, and Cav1.2.
  • Quantified auxiliary subunits Cavβ1 and Cavα2δ1 in the SDC insoluble fraction.
  • Demonstrated higher sensitivity for large membrane proteins compared to global proteomics.

Conclusions:

  • Developed a targeted proteomic method for systematic quantification of cardiac ion channels.
  • Absolute quantities (copy numbers) enable cross-study comparisons and biological insights.
  • This approach advances the study of ion channelopathies and cardiac electrical function.