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Updated: Jul 4, 2026

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Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays
Published on: August 26, 2022
Novel imaging approaches for visualizing root-mycorrhizal fungal interactions
Henry W G Birt1,2, Stephen J Paisey3, Patrick Möhl1,2
1Department of Earth and Environmental Sciences, The University of Manchester, Michael Smith Building, Manchester M13 9PL, UK.
Journal of Experimental Botany
|July 3, 2026
Summary
Advanced imaging techniques are revolutionizing the study of mycorrhizal fungi and plant root symbioses. These methods enable detailed visualization and analysis of nutrient exchange and structural colonization, improving our understanding of plant health and ecosystems.
Area of Science:
- Plant Biology
- Mycology
- Ecology
Background:
- Mycorrhizal fungi form crucial symbiotic relationships with plant roots, vital for nutrient exchange and plant health.
- Understanding these complex interactions requires advanced imaging capable of subcellular resolution and large-scale analysis.
Purpose of the Study:
- To review novel imaging approaches revolutionizing the study of root-mycorrhizal fungal symbioses.
- To highlight technologies enabling visualization of nutrient exchange and structural colonization.
Main Methods:
- X-ray computed tomography (micro-CT), X-ray fluorescence (XRF), and X-ray absorption near edge structure (XANES) spectroscopy for 3D visualization.
- Positron emission tomography (PET), fluorescent nanoparticles (FNPs), and electrical signaling monitoring for metabolic processes.
- AI-powered image processing for high-throughput analysis of complex imaging data.
Main Results:
- Non-destructive 3D visualization and characterization of mycorrhizal fungi and root structures are now possible.
- Metabolic processes and nutrient exchange can be tracked using PET, FNPs, and electrical signaling.
- In-field tracking at multiple scales is achievable with hyperspectral imaging and portable MRI.
Conclusions:
- Converging imaging technologies allow direct, continuous measurement of structural and metabolic root-mycorrhizal interactions.
- These advancements pave the way for a mechanistic understanding of symbiotic partnerships and their impact on plant health and ecosystems.

