Cell surface display for nutritional chemicals: Strategies, mechanisms, and evaluation methods.

Area of Science:

• Biotechnology
• Molecular Biology
• Industrial Microbiology

Background:

• Cell surface display is a key biotechnological tool for anchoring proteins on microbial cells.
• It enhances biocatalysis by enabling enzyme-substrate access without cell lysis or purification.

Purpose of the Study:

• To systematically review cell surface display technology.
• To cover display strategies, regulatory mechanisms, evaluation methods, and applications.

Main Methods:

• Discussion of host microorganisms.
• Summary of strategies for display efficiency (transcriptional regulation, secretion, anchoring, catalysis).
• Systematic review of evaluation methods for cell surface display systems.

Main Results:

• Comprehensive overview of cell surface display strategies and host systems.
• Detailed discussion on improving display efficiency across multiple aspects.
• Systematic evaluation of methods for assessing display systems.

Conclusions:

• Cell surface display offers a valuable framework for designing and deploying systems in industrial biotechnology.
• Applications span production of functional sugars, lipids, nucleotides, and nutritional compounds.