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Related Experiment Video

Updated: Jul 7, 2026

Characterizing Extracellular Vesicles from Biological Fluids
05:07

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

Investigation Into the Collection and Isolation Workflows of Extracellular Vesicles From Tears.

Nikolay Boychev1, Mudigunda V Sushma1, Vincent Yeung1

  • 1Department of Ophthalmology Harvard Medical School/Massachusetts Eye and Ear/Schepens Eye Research Institute Boston Massachusetts USA.

Journal of Extracellular Biology
|July 6, 2026
PubMed
Summary

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Contact lenses (CLs) and Schirmer strips (SS) offer distinct tear extracellular vesicle (tEV) profiles. CLs yield cleaner tEVs, while SS capture more cellular debris and inflammatory markers, informing biomarker discovery.

Area of Science:

  • Ophthalmology
  • Biomarker Discovery
  • Extracellular Vesicle Research

Background:

  • Tear extracellular vesicles (tEVs) are valuable biomarkers for ocular and systemic diseases.
  • Standardized methods for tEV collection and isolation are lacking.
  • Contact lenses (CLs) present a practical method for in-situ tear sampling.

Purpose of the Study:

  • To compare tear collection using CLs versus Schirmer Strips (SS).
  • To evaluate EV isolation techniques: ExoQuick (EQ) and ultracentrifugation (UC).
  • To characterize tEVs for biomarker potential.

Main Methods:

  • Comparison of CLs and SS for tear collection in rabbit eyes (n=4).
  • Isolation of tEVs using EQ polymer precipitation and UC.
  • Characterization of tEVs via particle counts, protein content, zeta potential, mRNA, and tetraspanins.
Keywords:
RNASchirmer stripsbiomarkerscontact lensesextracellular vesicleshousekeeping genestear fluid

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Last Updated: Jul 7, 2026

Characterizing Extracellular Vesicles from Biological Fluids
05:07

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

Extraction of Extracellular Vesicles from Whole Tissue
09:03

Extraction of Extracellular Vesicles from Whole Tissue

Published on: February 7, 2019

Optimizing Tear Collection in Mice for mRNA and Protein Analysis
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Optimizing Tear Collection in Mice for mRNA and Protein Analysis

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Main Results:

  • CL-derived tEVs had lower protein contamination and inflammatory cytokines.
  • SS-derived tEVs contained more cellular debris and inflammatory markers (MMP-9, TNF-α, Leptin).
  • EQ showed higher particle recovery but altered vesicle surface charge compared to UC.

Conclusions:

  • CLs and SS provide complementary tEV molecular information.
  • EQ and UC have different impacts on tEV recovery and stability.
  • This study establishes a framework for standardized tEV profiling.