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Updated: Jul 7, 2026

From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
Published on: January 13, 2017
Extending the starter-unit selection of a collaborating non-reducing polyketide synthase through rational domain
Ruya Yin1, Mengwei Zhang1, Ziqi Zhai1
1State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University Beijing 100193 China dwlai@cau.edu.cn.
None:
Fungal non-reducing polyketide synthases (NR-PKSs) use starter-unit acyl transferase (SAT) for selection of the starter unit. Here, we swapped a C6-loading SAT of the sorbicillin NR-PKS with an acetyl-loading SAT, resulting in the generation of non-native aromatic polyketides with a shorter chain length. This extends the starter-unit selection of a collaborating NR-PKS to C2. A general rule was summarized to predict the product chain-length after SAT-exchange. This study provides new insights towards the chain-length control in NR-PKS programming.
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