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Related Experiment Video

Updated: Jul 8, 2026

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
09:02

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Published on: June 11, 2020

Assessing Transduction Efficiency And Cell Targeting By Cell-penetrating Peptides In The Mouse Lung.

Jin Wen1, Rachel M Gilbert2, Daniel J Tschumperlin2

  • 1Department of Cardiovascular Medicine, Mayo Clinic.

Journal of Visualized Experiments : Jove
|July 6, 2026
PubMed
Summary
This summary is machine-generated.

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American journal of respiratory cell and molecular biology·2026

This study presents a new workflow to evaluate cell-penetrating peptides (CPPs) targeting specific lung cells. The method confirms CPP delivery to alveolar epithelial type II cells in mice.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Cell-penetrating peptides (CPPs) facilitate cellular uptake of large molecules.
  • In vivo cellular selectivity of CPPs requires cell-type specific validation.
  • Alveolar epithelial type II (AT2) cells are crucial for lung function and disease.

Purpose of the Study:

  • To develop and validate an integrated workflow for assessing CPP transduction efficiency in specific lung cell types.
  • To evaluate the in vivo cellular targeting of a cyclized CPP (cR11A) in mouse AT2 cells.

Main Methods:

  • Intravenous injection of Cy5.5-labeled cR11A in mice.
  • Paired-fraction fluorescence-activated cell sorting (FACS) of lung single cells.
  • Single-cell RNA sequencing.

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Protein Transfection of Mouse Lung
04:21

Protein Transfection of Mouse Lung

Published on: May 15, 2013

Related Experiment Videos

Last Updated: Jul 8, 2026

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide
09:02

In Vivo Imaging of Transduction Efficiencies of Cardiac Targeting Peptide

Published on: June 11, 2020

Protein Transfection of Mouse Lung
04:21

Protein Transfection of Mouse Lung

Published on: May 15, 2013

  • Flow cytometry in AT2 reporter mice (Sftpc-CreERT2;LSL-GFP).
  • Confocal microscopy of lung cryosections.

    • Main Results:

    • Concordant evidence of cR11A-Cy5.5 accumulation in AT2 cells at 15 and 60 minutes post-injection.
    • Successful isolation and analysis of CPP-positive and negative lung cell populations.
    • Confirmation of CPP colocalization with AT2 cell markers.

    Conclusions:

    • The presented integrated workflow reliably quantifies CPP transduction efficiency in a cell-type specific manner.
    • This framework provides a reproducible method for evaluating CPPs for targeted drug delivery to AT2 cells.
    • The workflow can be adapted for assessing CPP targeting in other organs.