Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic diversity of Listeria monocytogenes isolates in Colombia from 2010 until 2017 and comparison against the

Paula Lucía Díaz1, Julieth Carolina Gamba1, Diego Prada1

  • 1Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, D.C., Colombia.

Biomedica : Revista Del Instituto Nacional De Salud
|July 7, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrent meningitis due to <i>Salmonella arizonae</i> in an adult with AIDS: case presentation and next-generation sequencing analysis.

ASM case reports·2025
Same author

Genomic epidemiology of Colombian resistant <i>Candida auris</i> isolates.

Antimicrobial agents and chemotherapy·2025
Same author

Positivity analysis of <i>Bordetella</i> spp. and SARS-CoV-2 coinfections in clinical samples from Colombia, 2021-2022.

Microbiology spectrum·2025
Same author

Investigation of mobile genetic elements and their association with antibiotic resistance genes in clinical pathogens worldwide.

PloS one·2025
Same author

Draft genome sequences of <i>Bordetella parapertussis</i> clinical isolates during an increase in confirmed cases from Colombia, 2023.

Microbiology resource announcements·2025
Same author

Global diversity and antimicrobial resistance of typhoid fever pathogens: Insights from a meta-analysis of 13,000 <i>Salmonella</i> Typhi genomes.

eLife·2023
This summary is machine-generated.

This study characterized Listeria monocytogenes clinical isolates in Colombia from 2010-2017. Genomic and phenotypic analysis revealed key virulence factors and antimicrobial resistance, informing public health control strategies for this foodborne illness.

Area of Science:

  • Microbiology
  • Genomics
  • Epidemiology

Background:

  • Listeriosis, caused by Listeria monocytogenes, is a foodborne illness with high mortality in immunocompromised individuals.
  • Listeriosis is not a mandatory reportable disease in Colombia, limiting surveillance.
  • Characterizing clinical L. monocytogenes isolates is crucial for understanding disease dynamics and improving control.

Purpose of the Study:

  • To phenotypically and genotypically characterize clinical isolates of Listeria monocytogenes from Colombia between 2010 and 2017.
  • To identify antimicrobial susceptibility patterns, serotypes, and genetic relatedness of isolates.
  • To inform public health interventions by understanding the molecular epidemiology of L. monocytogenes in Colombia.

Main Methods:

  • Analysis of 63 L. monocytogenes isolates from laboratory surveillance data.
Keywords:
Listeria monocytogeneselectroforesis en gel de campo pulsadoenfermedades transmitidas por los alimentoslisteriosismeningitismortalidad perinatalresistencia a medicamentossecuenciación completa del genoma

Related Experiment Videos

  • Antimicrobial susceptibility testing, serotyping (O-H antigens), and Polymerase Chain Reaction (PCR).
  • Pulsed-field gel electrophoresis (PFGE-ApaI) and whole-genome sequencing (WGS) including multilocus sequence typing (MLST) and ribosomal MLST (rMLST).

    • Main Results:

    • Most isolates were from females (50.7%) and the 15-54 age group (33.3%), primarily from blood cultures (58.7%).
    • Isolates showed sensitivity to penicillin and trimethoprim-sulfamethoxazole. Serogroup 4b-4d-4e predominated (77.8%).
    • WGS identified sequence types (ST1, ST2), clonal complexes (CC1, CC2), lineage I, serotype 4b, virulence genes (LIPI-1, LIPI-3), resistance genes (fosX, mprF), plasmids, and prophages. Global phylogenetic analysis linked isolates to lineage I strains from other countries.

    Conclusions:

    • Strengthening epidemiological surveillance and molecular typing of L. monocytogenes is essential for effective public health control.
    • Genomic characterization provides valuable insights into virulence, resistance, and transmission dynamics.
    • This study provides a baseline for understanding L. monocytogenes in Colombia and guides future public health strategies.