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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...

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Related Experiment Video

Updated: Jul 9, 2026

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Stretching the limits: Decoding hidden biology with vibrational probes.

Ziguang Yang1, Philip A Kocheril1, Adrian Colazo1

  • 1Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.

Current Opinion in Chemical Biology
|July 7, 2026
PubMed
Summary
This summary is machine-generated.

Vibrational bioimaging uses specialized probes for detailed biological studies. Recent advancements enable highly specific imaging of metabolic processes and probe engineering for advanced applications.

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A Novel Platform for In Vitro Cellular Stretching and Imaging
07:38

A Novel Platform for In Vitro Cellular Stretching and Imaging

Published on: March 10, 2026

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Last Updated: Jul 9, 2026

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

A Novel Platform for In Vitro Cellular Stretching and Imaging
07:38

A Novel Platform for In Vitro Cellular Stretching and Imaging

Published on: March 10, 2026

Area of Science:

  • Biophysics
  • Chemical Biology
  • Molecular Imaging

Background:

  • Vibrational bioimaging has become a key technique for analyzing biological systems over the last 20 years.
  • Initial research focused on label-free approaches, but vibrational probes have significantly enhanced specificity.
  • Vibrational probes allow for the study of metabolic incorporation and turnover with high precision.

Purpose of the Study:

  • To review recent advancements in the application of vibrational probes for biological imaging.
  • To explore the potential of probe engineering for developing novel vibrational imaging techniques.
  • To provide insights into future directions for functional studies of biological processes.

Main Methods:

  • Review of literature on vibrational bioimaging and probes.
  • Analysis of probe engineering strategies for enhanced specificity and sensitivity.
  • Discussion of applications in metabolic imaging and quantitative sensing.

Main Results:

  • Vibrational probes have enabled highly specific imaging of metabolic incorporation and turnover.
  • Probe engineering has led to advancements in single-molecule imaging and super-multiplex imaging.
  • Quantitative vibrational local sensing capabilities have been developed.

Conclusions:

  • Vibrational probes are crucial for advanced biological imaging and functional studies.
  • Continued probe engineering will unlock deeper insights into biological dynamics.
  • Future research should focus on designing novel probes for complex biological processes.