Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.

Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ultrasensitive electrochemical dipstick lateral flow immunoassay for extracellular vesicle quantification using starch-iodine functionalized gold nanoparticles.

Mikrochimica acta·2026

Same author

Lycopene-Loaded Emulsions: Chitosan Versus Non-Ionic Surfactants as Stabilizers.

Molecules (Basel, Switzerland)·2024

Same author

Esterification of dextran by octenyl succinic anhydride (OSA): Physicochemical characterization and functional properties assessment.

Carbohydrate polymers·2024

Same author

Phage Lytic Protein CHAPSH3b Encapsulated in Niosomes and Gelatine Films.

Microorganisms·2024

Same author

Characterization of Pickering emulsions stabilized by delipidated egg yolk granular protein nanoparticles crosslinked with ultraviolet radiation.

Food chemistry·2023

Same author

Rapid and sensitive detection of E. coli O157:H7 by lateral flow immunoassay and silver enhancement.

Mikrochimica acta·2023

Search research articles

Related Experiment Video

Updated: Jul 10, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Starch-based labelling for electrochemical lateral flow immunoassays.

Clara Saweres-Argüelles¹, Alberto Sánchez-Calvo², Gemma Gutiérrez³

¹Department of Physical and Analytical Chemistry and Institute of Biotechnology of Asturias, University of Oviedo, Julián Clavería 8, Oviedo, Asturias 33006, Spain; Department of Chemical and Environmental Engineering and Institute of Biotechnology of Asturias, University of Oviedo, Julián Clavería 8, Oviedo, Asturias 33006, Spain.

Colloids and Surfaces. B, Biointerfaces

|July 8, 2026

Summary

Researchers developed eco-friendly nanolabels for electrochemical lateral flow immunoassays (eLFIA). Lipid-polymer micelles encapsulating a starch-polyiodide complex achieved sensitive detection, paving the way for sustainable diagnostics.

Keywords:

Electrochemical lateral flow immunoassays Micelles Starch Supramolecular nanostructures Sustainable nanomaterials Vesicles

More Related Videos

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Related Experiment Videos

Last Updated: Jul 10, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection

Published on: October 23, 2009

Area of Science:

Nanotechnology
Electrochemistry
Biomedical Diagnostics

Background:

Electrochemical Lateral Flow Immunoassays (eLFIA) offer sensitive, portable, and affordable in vitro diagnostics.
A key challenge in eLFIA development is creating non-toxic, biodegradable nanolabels for sensitive quantification.

Purpose of the Study:

To synthesize and characterize novel nanocarriers (liposomes, niosomes, lipid-polymer micelles) for eLFIA.
To encapsulate a starch-polyiodide complex within these nanocarriers to serve as an electrochemical probe.
To establish a proof-of-concept for a sustainable eLFIA using bio-derived nanoparticles.

Main Methods:

Synthesis and characterization of liposomes, niosomes, and lipid-polymer micelles.
Encapsulation of starch-polyiodide complex within nanocarriers.
Evaluation of nanocarrier properties: size, morphology, molecular structure, encapsulation efficiency, and colloidal stability.
Proof-of-concept eLFIA using biotin-neutravidin model system with fixed-potential coulometry.

Main Results:

Lipid-polymer micelles were identified as the optimal nanolabel due to favorable characteristics.
The developed eLFIA system demonstrated high sensitivity, reaching a detection limit of 17 nM.
The total assay time was optimized to just over 20 minutes, including a 3-minute fixed-potential coulometry step.

Conclusions:

This study presents the first in vitro diagnostic device utilizing sustainable, bio-derived nanoparticles for eLFIA.
The developed lipid-polymer micelle-based nanolabels offer a promising platform for sensitive and environmentally friendly diagnostic tests.
This work paves the way for the future development of greener diagnostic tools.