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Compartment-Specific Variation in Bacterial Microbiome and Polyphyllin Profiles in Paris polyphylla
Xinhong Wu1, Yan Deng2, Shihui Li1
1School of Resource Processing and Bioengineering, Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha, China, csu.edu.cn.
International Journal of Microbiology
|July 9, 2026
Summary
This study reveals how bioactive polyphyllins in Paris polyphylla influence its bacterial microbiome across different plant parts. Higher polyphyllin levels shape unique microbial communities, impacting plant health and cultivation.
Area of Science:
- Plant-microbe interactions
- Medicinal plant research
- Microbiome analysis
Background:
- Paris polyphylla is a key traditional Chinese medicinal plant.
- Spatial distribution of its bacterial microbiome and polyphyllins is poorly understood.
- Understanding these relationships is crucial for optimizing cultivation.
Purpose of the Study:
- To investigate bacterial microbiome and polyphyllin distribution across Paris polyphylla compartments.
- To explore correlations between polyphyllins and bacterial communities.
- To validate plant-microbe interactions experimentally.
Main Methods:
- 16S rRNA amplicon sequencing
- Metabolite analysis
- Bioinformatics
- Spot inoculation assays
Main Results:
- Polyphyllins showed compartment-specific accumulation (e.g., aerial vs. root).
- Bacterial diversity decreased from soil to leaves, driven by niche differentiation and polyphyllins.
- Pseudomonas abundance correlated inversely with polyphyllin I and positively with polyphyllin VII.
- Polyphyllin content significantly influenced bacterial community composition.
Conclusions:
- Paris polyphylla exhibits selective enrichment of beneficial microbes across compartments.
- Polyphyllins play a significant role in shaping the plant's bacterial microbiome.
- Findings provide a basis for optimizing cultivation and developing microbial inoculants.
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