Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hydrothermal Humification for Producing Humic-like Acids and Nitrogen-Enriched Humic-like Acids from Recycled Wheat Straw CTMP Black Liquor.

Polymers·2026

Same author

Vacuum Pyrolysis Engineered CoSb/C Scaffold for Sodium Metal Anodes with Sodiophilic and Superionic Interphase.

Nano letters·2026

Same author

Confronting the known unknown: historical lessons and future strategies for Disease X.

Frontiers in public health·2026

Same author

Impact of compost amendments and Morus alba L. on soil restoration, microbial communities, and crop yield in rare-earth tailings.

Ecotoxicology and environmental safety·2026

Same author

Far-Red Light Regulates the Circadian Rhythm Pathway to Accelerate Rice Flowering.

International journal of molecular sciences·2026

Same author

Dual-Gradient Structural Design for Synchronized Phase Transitions and Stress-Resilient O3-Type Sodium Cathodes.

ACS nano·2026

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Molecular characterization of cell dynamics during osteosarcoma progression.

Xiangjie Li¹, Junjie Liu¹, Yu Wang¹

¹Wuxi School of Medicine, Jiangnan University, Wuxi, 214000, PR China; Department of Orthopedics, Affiliated Hospital of Jiangnan University, Wuxi, 214000, PR China.

Translational Oncology

|July 9, 2026

Summary

Osteosarcoma metastasis involves significant microenvironment remodeling, with altered cell proportions and immune cell reprogramming. This study provides a comprehensive view of cellular changes during osteosarcoma progression.

Keywords:

Cell differentiation trajectories Osteosarcoma Spatial transcriptomics Tumor metastasis UMAP

More Related Videos

Methods to Enable Spatial Transcriptomics of Bone Tissues

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Related Experiment Videos

Last Updated: Jul 12, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

Methods to Enable Spatial Transcriptomics of Bone Tissues

Methods to Enable Spatial Transcriptomics of Bone Tissues

Published on: May 3, 2024

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells

Published on: May 6, 2018

Area of Science:

Oncology
Genomics
Immunology

Background:

Osteosarcoma is a common bone cancer with high heterogeneity and complex metastatic processes.
Current treatments face challenges due to tumor heterogeneity and dynamic cellular changes during metastasis.

Purpose of the Study:

To systematically analyze cellular composition and microenvironment dynamics during osteosarcoma progression from primary to metastatic stages.
To integrate multi-center single-cell and spatial transcriptomics data for a comprehensive understanding of osteosarcoma evolution.

Main Methods:

Single-cell RNA sequencing (scRNA-seq) with SingleR for cell annotation and subcluster analysis.
Pseudotime analysis using Monocle2 and scTour to construct cell differentiation trajectories.
Spatial transcriptomics deconvolution with CARD to analyze cell distribution.

Main Results:

Metastatic osteosarcoma shows significant microenvironment remodeling: increased fibroblasts, mast cells, T/NK cells; decreased osteoblasts, myeloid cells.
Identified YY1 as a spatial regulator of IER5L in subtype 2, linked to divergent evolutionary paths.
Observed immune and stromal cell reprogramming, including functional transformation of tumor-associated macrophages (TAMs) in metastatic foci.

Conclusions:

This study represents a large-scale integration of multi-center data covering primary, recurrent, and metastatic osteosarcoma.
It systematically depicts dynamic cellular changes, offering insights into osteosarcoma progression and metastasis.
Findings highlight the potential for novel therapeutic strategies targeting the evolving tumor microenvironment.