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UFD1 facilitates GAstV-II replication in GEF cells via suppressing type I interferon production
Zeng Wang1, Saimin Zhai2, Huichao Gao1
1College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
Microbial Pathogenesis
|July 9, 2026
Summary
Goose UFD1 protein promotes goose astrovirus II (GAstV-II) replication by suppressing type I interferon production. This study reveals UFD1 as a key factor in GAstV-II infection, impacting goose health.
Area of Science:
- Virology
- Immunology
- Molecular Biology
Background:
- Goose astrovirus II (GAstV-II) causes gout in goslings, significantly impacting the goose industry.
- Ubiquitin recognition factor in ER-associated degradation protein 1 (UFD1) is vital for proteostasis via the ER-associated degradation (ERAD) pathway.
Purpose of the Study:
- To clone and characterize the goose UFD1 gene.
- To investigate the role of goose UFD1 in GAstV-II infection.
Main Methods:
- Cloning of the goose UFD1 gene.
- Analysis of UFD1 expression in goose embryo fibroblasts (GEFs) post-GAstV-II infection.
- Overexpression and knockdown experiments of UFD1 in GEFs.
- Assessment of type I interferon (IFN-I) production.
Main Results:
- Goose UFD1 shows high homology with its duck ortholog.
- GAstV-II infection upregulates UFD1 protein expression in GEFs.
- UFD1 overexpression enhances GAstV-II replication, while UFD1 knockdown inhibits it.
- UFD1 suppresses IFN-I production, thereby supporting GAstV-II replication.
Conclusions:
- Goose UFD1 acts as a positive regulator of GAstV-II replication.
- UFD1 facilitates viral replication by inhibiting host type I interferon response.
- This study elucidates a novel molecular interaction between GAstV-II and host cells.
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