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Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

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Published on: November 20, 2018

Live-Cell SICM Imaging: An Introductory Guide for New Users.

Martina Papa^1,2, François Treussart², Jean-Pierre Mothet²

¹École Polytechnique, Institut Polytechnique de Paris, CNRS, LPICM, 91128 Palaiseau, France.

Analytical Chemistry

|July 9, 2026

Summary

Scanning ion conductance microscopy (SICM) provides high-resolution, minimally invasive live-cell imaging. This guide details SICM implementation for studying cellular structure and dynamics.

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Area of Science:

Cell Biology
Biophysics
Microscopy Techniques

Background:

High-resolution imaging is crucial for understanding cellular morphology and dynamics.
Live-cell imaging requires techniques that maintain physiological conditions.
Existing methods may lack the resolution or non-invasiveness needed for detailed live-cell studies.

Purpose of the Study:

To provide a practical guide for implementing Scanning Ion Conductance Microscopy (SICM) for live-cell imaging.
To equip researchers with the knowledge for successful SICM experiments and data analysis.
To facilitate the exploration of live-cell structure and dynamics using SICM.

Main Methods:

Detailed instrumentation setup for SICM.
Probe characterization and selection protocols.
Best practices for live-cell preparation and sample handling.
Methods for morphometric data extraction and quantitative analysis.
Guidelines for data presentation in live-cell imaging studies.

Main Results:

SICM enables nanoscale topographic imaging of live cells.
Experimental conditions compatible with realistic live-cell behavior are maintained.
The guide covers essential steps from instrumentation to data presentation.
Researchers are empowered to confidently use SICM for live-cell investigations.

Conclusions:

SICM is a powerful tool for high-resolution, minimally invasive live-cell imaging.
This guide offers a comprehensive framework for SICM application.
SICM facilitates detailed studies of live-cell structure and dynamic processes.