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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
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Utilizing Magnetic Microraft Technology for Single-Cell Isolation of CTCs from Whole Blood for scRNA-Seq

Terri Clister1, Rosalyn M Fey1, Zachary R Garrison1

  • 1Department of Dermatology, Oregon Health & Science University, Portland, OR, 97239, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 9, 2026
PubMed
Summary

Researchers developed a new method to detect circulating tumor cells (CTCs) in blood using positive and negative enrichment. This technique enables full-length single-cell RNA sequencing of CTCs for improved cancer prognosis.

Keywords:
Circulating tumor cells (CTCs)MicroraftsSingle cellTranscriptomic analysissc-RNAseq

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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment

Published on: May 14, 2019

Area of Science:

  • Oncology
  • Molecular Biology
  • Biotechnology

Background:

  • Circulating tumor cells (CTCs) are vital biomarkers for cancer prognosis.
  • Current CTC isolation methods using cell surface markers limit the analysis of cellular heterogeneity.

Purpose of the Study:

  • To develop an improved method for detecting disease-specific CTCs in peripheral blood.
  • To enable full-length single-cell RNA sequencing of CTCs for comprehensive analysis.

Main Methods:

  • Employed both positive and negative enrichment strategies for CTC isolation.
  • Utilized the Smart-seq3 library preparation protocol for full-length transcript sequencing.
  • Performed single-cell RNA sequencing on isolated melanoma cells spiked into healthy blood.

Main Results:

  • Successfully isolated melanoma cells from healthy blood samples.
  • Demonstrated the capability of the new method for CTC detection and analysis.
  • Enabled full-length transcript sequencing at the single-cell level for CTCs.

Conclusions:

  • The developed method offers a more comprehensive approach to CTC isolation and analysis.
  • This technique enhances the potential of CTCs as biomarkers for cancer prognosis.
  • Single-cell RNA sequencing of CTCs provides deeper insights into tumor heterogeneity and biology.