Related Experiment Video
Updated: Jul 12, 2026
Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
Published on: May 12, 2023
A Simple, Cost-Effective, High-Throughput Method for Measuring Chromatin Accessibility and Gene Expression in Single
Zhifei Luo1, William J Greenleaf1,2
1Department of Genetics, Stanford University, Stanford, CA, USA.
Abstract:
We describe microfluidic-free, droplet-based methods for single-nucleus epigenomic measurements: Particle-templated Instant Partition single-nucleus assay for transposase-accessible chromatin using sequencing (PIP-ATAC-seq) and its multiomic version (PIP-Multiome-seq). We benchmarked these assays by generating data sets containing thousands of nuclei using cell lines and mouse brains and compared to other established methods. PIP-Multiome and PIP-ATAC are straightforward to implement, affordable, and produce high-quality data, providing useful additions to the single-cell molecular measurement armamentarium.
