Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An encyclopedia of human enhancer-gene regulatory interactions.

Nature·2026
Same author

Epigenetic landscape, key transcriptional regulators, and in vivo identification of human Tr1 cells.

Science advances·2026
Same author

Intrinsic promoter responsiveness dictates sensitivity to transcriptional activation by enhancers.

bioRxiv : the preprint server for biology·2026
Same author

Prediction and functional interpretation of inter-chromosomal genome architecture from DNA sequence with TwinC.

Nature communications·2026
Same author

Multiomics and deep learning dissect regulatory syntax in human development.

Nature·2026
Same author

Genetics and environment distinctively shape the human immune cell epigenome.

Nature genetics·2026

Related Experiment Video

Updated: Jul 12, 2026

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
10:03

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

Published on: May 12, 2023

A Simple, Cost-Effective, High-Throughput Method for Measuring Chromatin Accessibility and Gene Expression in Single

Zhifei Luo, William J Greenleaf

    Biorxiv : the Preprint Server for Biology
    |July 10, 2026
    PubMed
    Summary

    New droplet-based methods, Particle-templated Instant Partition (PIP-ATAC-seq) and PIP-Multiome-seq, offer affordable, high-quality single-nucleus epigenomic measurements without microfluidics.

    More Related Videos

    Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
    08:08

    Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

    Published on: April 2, 2018

    Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
    09:20

    Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

    Published on: February 1, 2022

    Related Experiment Videos

    Last Updated: Jul 12, 2026

    Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
    10:03

    Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

    Published on: May 12, 2023

    Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells
    08:08

    Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells

    Published on: April 2, 2018

    Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
    09:20

    Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

    Published on: February 1, 2022

    Area of Science:

    • Molecular Biology
    • Genomics
    • Biotechnology

    Background:

    • Single-nucleus epigenomic profiling is crucial for understanding cellular heterogeneity.
    • Existing methods often rely on microfluidics, which can be complex and costly.
    • There is a need for accessible and efficient techniques for large-scale epigenomic analysis.

    Purpose of the Study:

    • To introduce novel microfluidic-free, droplet-based assays for single-nucleus epigenomic profiling.
    • To present Particle-templated Instant Partition single-nucleus assay for transposase-accessible chromatin using sequencing (PIP-ATAC-seq) and its multiomic counterpart (PIP-Multiome-seq).
    • To evaluate the performance and utility of these new assays.

    Main Methods:

    • Development of droplet-based, microfluidic-free assays: PIP-ATAC-seq and PIP-Multiome-seq.
    • Generation of large-scale datasets from thousands of nuclei using cell lines and mouse brains.
    • Benchmarking and comparison against established single-nucleus epigenomic measurement techniques.

    Main Results:

    • PIP-ATAC-seq and PIP-Multiome-seq successfully generated high-quality epigenomic data from thousands of nuclei.
    • The assays demonstrated straightforward implementation and affordability.
    • Performance was comparable to existing, more complex methods.

    Conclusions:

    • PIP-ATAC-seq and PIP-Multiome-seq are valuable, cost-effective additions to the single-cell molecular measurement toolkit.
    • These methods provide accessible approaches for high-throughput single-nucleus epigenomic and multiomic profiling.
    • The microfluidic-free design enhances the practicality and scalability of these assays.