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Related Experiment Video

Updated: Jul 12, 2026

Serial Two-Photon Tomography of the Whole Marmoset Brain for Neuroanatomical Analyses
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Published on: January 17, 2025

Genetically Encoded Melanin as a Photostable Scattering Contrast for Whole-Brain Tomography.

Peilin Gu, Chong Chen, Jian Ren

    Biorxiv : the Preprint Server for Biology
    |July 10, 2026
    PubMed
    Summary

    We developed MelaCAST, a new method using melanin for whole-brain imaging in mice. This non-photobleaching technique enables stable, quantitative analysis of genetically targeted cells in intact tissues.

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    Simultaneous Evaluation of Cerebral Hemodynamics and Light Scattering Properties of the In Vivo Rat Brain Using Multispectral Diffuse Reflectance Imaging

    Published on: May 7, 2017

    Area of Science:

    • Neuroscience
    • Biophysics
    • Biotechnology

    Background:

    • Traditional fluorescence imaging faces limitations like photobleaching and signal inconsistency in large-scale brain studies.
    • Quantitative analysis of intact tissues is challenging with current imaging modalities.

    Purpose of the Study:

    • To introduce MelaCAST (melanin-based scattering CAST imaging), a novel genetically encoded scattering method for whole-brain visualization.
    • To overcome the limitations of fluorescence imaging for high-throughput, quantitative analysis of intact brain tissues.

    Main Methods:

    • Utilized adeno-associated virus (AAV) to deliver tyrosinase for cell-type-specific melanin synthesis.
    • Combined advanced tissue clearing techniques with scattering tomography.
    • Developed melanin as an intracellular scattering contrast agent.

    Main Results:

    • MelaCAST provides stable, non-photobleaching intracellular scattering contrast across the entire mouse brain.
    • Enabled high-throughput, volumetric imaging of genetically targeted cell populations in intact brains.
    • Demonstrated melanin's efficacy as a genetically encoded scattering reporter.

    Conclusions:

    • MelaCAST establishes a new paradigm for whole-organ imaging, moving beyond traditional fluorescence methods.
    • The technique offers a robust solution for quantitative, large-scale neural circuit analysis.
    • Melanin-based scattering imaging significantly broadens capabilities in neuroscience research.