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Related Experiment Video

Updated: Jul 12, 2026

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry
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Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Published on: March 7, 2019

High-throughput whole-brain scattering imaging resolves amyloid plaques through clearing-assisted contrast

Chong Chen, Peilin Gu, Jian Ren

    Biorxiv : the Preprint Server for Biology
    |July 11, 2026
    PubMed
    Summary

    We developed clearing-assisted scattering tomography (CAST) for high-throughput, label-free whole-brain imaging. This method enhances scattering contrast in cleared tissue, enabling detailed mapping of amyloid plaques and brain structures in Alzheimer's disease models.

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    Last Updated: Jul 12, 2026

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    07:47

    Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases

    Published on: December 6, 2016

    Area of Science:

    • Biomedical Imaging
    • Neuroscience
    • Pathology

    Background:

    • Label-free scattering imaging offers sensitive tissue assessment without contrast agents.
    • Limited optical penetration hinders its application in whole-organ pathology.
    • Existing methods struggle with deep tissue imaging and contrast for specific pathologies.

    Purpose of the Study:

    • To develop a high-throughput, label-free imaging method for whole-brain pathology.
    • To overcome the optical penetration limitations of traditional scattering imaging.
    • To enable system-level analysis of tissue architecture and amyloid pathology in Alzheimer's disease models.

    Main Methods:

    • Introduced clearing-assisted scattering tomography (CAST).
    • Utilized selective lipid clearance for scattering enhancement (SELiC) to modulate refractive index heterogeneity.
    • Enabled whole-brain optical penetration in cleared mouse brains.

    Main Results:

    • CAST provides whole-brain optical penetration while maintaining strong scattering contrast.
    • Successfully imaged intact mouse brains and mapped amyloid plaques brain-wide.
    • Demonstrated visualization of white-matter fibre bundles alongside amyloid pathology.

    Conclusions:

    • CAST is a scalable platform for label-free, system-level analysis of Alzheimer's disease pathology.
    • The SELiC technique enhances scattering contrast in cleared tissues for improved imaging.
    • This approach facilitates comprehensive analysis of brain architecture and disease markers.