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Updated: Jul 12, 2026
Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells
Published on: June 7, 2019
Exploring the apoptotic potential of Prunus spinosa Trigno extract in BRAF-mutated melanoma cells
Alessia Di Pauli1,2, Rosa Vona3, Alice Di Netta2
1Department of Anatomy, Histology, Forensic-Medicine and Orthopedics, Section of Histology and Embryology, Sapienza University of Rome, Rome, 00161, Italy.
Background:
Melanoma is one of the most aggressive forms of cancer in human due to its ability to invade tissues and metastasize. The aim of this work is to examine the effect of our patented compound Prunus spinosa Trigno + Nutraceutical Activator Complex (PsT + NAC®) on primary (WM115), metastatic (WM266-4), and malignant (A375) human melanoma cell lines.
Methods And Results:
The data show that PsT + NAC® induced a dose- and time-dependent reduction in cell viability in all melanoma cell lines, particularly in the metastatic WM266-4. Persistent morphological changes indicative of cell death were observed, which remained irreversible even after the cells recovered from treatment. The treatment with PsT + NAC® altered cell migration and motility by remodeling of actin cytoskeleton. Cell cycle analysis revealed a G2/M phase arrest in the primary WM115 cells, and a G1 phase arrest-at lower concentrations-in the metastatic WM266-4 cells, and in the malignant A375 cells. As the treatment concentration increased, all melanoma cell lines showed an increase in the sub-G1 population, associated with apoptosis. Western blotting analysis revealed that lower concentrations of PsT + NAC® induced a protective autophagic response, while higher concentrations triggered caspase-dependent apoptosis.
Conclusion:
These results demonstrate the efficacy of PsT + NAC® in inhibiting the growth of BRAF-mutant melanoma cells.
Insights
The novel compound Prunus spinosa Trigno + Nutraceutical Activator Complex (PsT + NAC®) effectively reduced melanoma cell viability and induced cell death. This compound also altered cell migration and triggered apoptosis in BRAF-mutant melanoma cells.
Area of Science:
- Oncology
- Molecular Biology
- Pharmacology
Background:
- Melanoma is an aggressive cancer characterized by invasion and metastasis.
- Developing effective treatments for melanoma remains a critical challenge.
Purpose of the Study:
- To investigate the anti-melanoma effects of the patented compound Prunus spinosa Trigno + Nutraceutical Activator Complex (PsT + NAC®).
- To evaluate the impact of PsT + NAC® on primary, metastatic, and malignant human melanoma cell lines.
Main Methods:
- Cell viability assays were performed on WM115, WM266-4, and A375 melanoma cell lines.
- Morphological changes, cell migration, cell cycle, and apoptosis were analyzed.
- Western blotting was used to assess autophagic and apoptotic pathways.
Main Results:
- PsT + NAC® significantly reduced melanoma cell viability in a dose- and time-dependent manner.
- Treatment induced morphological changes indicative of irreversible cell death and altered cell migration.
- Cell cycle arrest (G2/M or G1) and apoptosis (sub-G1 increase) were observed, with higher concentrations triggering caspase-dependent apoptosis.
- Lower concentrations induced a protective autophagic response.
Conclusions:
- PsT + NAC® demonstrates significant efficacy in inhibiting the growth of BRAF-mutant melanoma cells.
- The compound induces cell death through apoptosis and modulates cellular processes like migration and cell cycle progression.
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