Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variable proton conductance of submitochondrial particles.

M C Sorgato, S J Ferguson

    Biochemistry
    |December 11, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ex vivo biomechanical evaluation of 2.4 mm LCP plate rod constructs versus 2.7 mm LCP applied to the feline tibia.

    Veterinary surgery : VS·2023
    Same author

    Physiological stretching induces a differential extracellular matrix gene expression response in acetabular labrum cells.

    European cells & materials·2022
    Same author

    Lateral stability in healthy proximal interphalangeal joints versus surface replacement and silicone arthroplasty: Results of a three-dimensional motion analysis study.

    Hand surgery & rehabilitation·2020
    Same author

    Nanofiber membranes as biomimetic and mechanically stable surface coatings.

    Materials science & engineering. C, Materials for biological applications·2020
    Same author

    Systemic sclerostin antibody treatment increases osseointegration and biomechanical competence of zoledronic-acid-coated dental implants in a rat osteoporosis model.

    European cells & materials·2019
    Same author

    Patient characteristics affect hip contact forces during gait.

    Osteoarthritis and cartilage·2019

    Oxidizing succinate in bovine heart mitochondria unexpectedly generated a higher protonmotive force than NADH oxidation. This suggests electron flow through respiratory site 1 may increase proton membrane conductance, challenging assumptions of constant passive proton flow.

    Area of Science:

    • Mitochondrial respiration
    • Bioenergetics
    • Cellular energy production

    Background:

    • The protonmotive force (PMF) is crucial for ATP synthesis in mitochondria.
    • Understanding the relationship between substrate oxidation rates and PMF is key to cellular energy metabolism.

    Purpose of the Study:

    • To investigate the relationship between substrate oxidation rates and the generated protonmotive force in bovine heart submitochondrial particles.
    • To explore the impact of electron transport chain activity on mitochondrial membrane proton conductance.

    Main Methods:

    • Utilized bovine heart submitochondrial particles.
    • Measured protonmotive force generation during oxidation of succinate and NADH.
    • Assessed proton translocation rates.

    Related Experiment Videos

  • Inhibited succinate oxidation with malonate to study PMF changes.

    • Main Results:

    • Succinate oxidation unexpectedly yielded a higher PMF than NADH oxidation.
    • Proton translocation was inferred to be lower with succinate despite higher PMF.
    • PMF remained stable during significant inhibition (>75%) of succinate oxidation by malonate.

    Conclusions:

    • Electron flow through respiratory site 1 may enhance mitochondrial membrane proton conductance.
    • A constant passive membrane conductance for protons cannot be assumed in mitochondrial bioenergetics.
    • These findings refine our understanding of respiratory control and energy transduction.