Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Bacteriophages

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Integrase-On-Demand: bioprospecting integrases for targeted genomic insertion of genetic cargo.

Nucleic acids research·2026

Same author

Exploring phage-host interactions in <i>Burkholderia cepacia</i> complex bacterium to reveal host factors and phage resistance genes using CRISPRi functional genomics and transcriptomics.

Microbiology spectrum·2025

Same author

The TIGER/Islander resource for genomic islands.

Microbiology resource announcements·2025

Same author

CRISPRi-ART enables functional genomics of diverse bacteriophages using RNA-binding dCas13d.

Nature microbiology·2025

Same author

Meeting report: International soil virus conference 2024.

Virus research·2025

Same author

Discovery of antimicrobial activity in chemical extracts derived from unexplored algal-bacterial culture systems and isolates.

Scientific reports·2024

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

HtPIP: High-throughput phage isolation platform increases diversity and reduces isolation time using multiple

Ben Diaz¹, Tessa House¹, Meghana Padala¹

¹Department of Bioengineering and Biotechnology, Sandia National Laboratories, Livermore, CA, United States.

Frontiers in Microbiology

|July 11, 2026

Summary

A new High-throughput Phage Isolation Platform (HtPIP) efficiently discovers novel bacteriophages from environmental samples. This method accelerates phage discovery for diverse bacterial hosts, advancing phage biotechnology applications.

Keywords:

high-throughput phage discovery phage diversity phage genome phages

More Related Videos

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

Related Experiment Videos

Last Updated: Jul 12, 2026

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries

Published on: January 17, 2015

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Published on: May 1, 2018

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Application of the Intelligent High-Throughput Antimicrobial Sensitivity Testing/Phage Screening System and Lar Index of Antimicrobial Resistance

Published on: July 21, 2023

Area of Science:

Microbiology
Virology
Biotechnology

Background:

Bacteriophages are crucial for biotechnology, but their discovery is limited.
Scaling phage isolation for diverse hosts is time-consuming and expensive.

Purpose of the Study:

To develop a high-throughput method for isolating novel bacteriophages.
To increase the diversity of phages available for biotechnological applications.

Main Methods:

Developed the High-throughput Phage Isolation Platform (HtPIP) using 0.2-μM filter plates.
Applied HtPIP to environmental samples for parallel phage capture from multiple bacterial strains.
Utilized metagenomic analysis to assess phage novelty.

Main Results:

Isolated 12 novel phages across 9 bacterial genera using HtPIP.
Discovered 11 new phage species and 9 new genera.
Identified DNA and RNA phages, including a novel RNA phage outside Proteobacteria.

Conclusions:

The HtPIP significantly enhances the efficiency and scope of phage discovery.
This platform accelerates the development of phage-based biotechnologies.
The discovered phages expand the toolkit for phage research and applications.