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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
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Stability of immature platelets present in platelet units during hemoconcentration

Matthew R Howlett1, Victoria E Cary1, Robert W Maitta2,3

  • 1Department of Pathology, University Hospitals Cleveland Medical Center, Cleveland, OH, USA.

Journal of Thrombosis and Thrombolysis
|July 11, 2026
PubMed

Insights

Routine blood manipulation, including centrifugation and hemoconcentration, preserves immature platelets in platelet units. These findings suggest that immature platelet counts and viability remain stable after standard processing.

Area of Science:

  • Hematology
  • Transfusion Medicine
  • Platelet Biology

Background:

  • Immature platelets are the youngest circulating platelets and are present in all transfused platelet units.
  • Limited data exists on the impact of routine blood manipulation on immature platelets in transfusion facilities.

Purpose of the Study:

  • To quantify changes in immature platelets during routine volume reduction procedures in platelet units.
  • To assess the impact of centrifugation and hemoconcentration on immature platelet counts and viability.

Main Methods:

  • Platelet units underwent centrifugation, volume reduction, and resuspension.
  • Quantitative analysis of total and immature platelet counts before and after manipulation.
  • Assessment of the ratio of platelets to immature platelets and immature platelet viability.

Main Results:

  • Centrifugation and hemoconcentration led to a significant increase in platelets and immature platelets per milliliter.
  • Total platelet and immature platelet counts were conserved post-manipulation.
  • The ratio of platelets to immature platelets remained unchanged, and immature platelet viability was unaffected.

Conclusions:

  • Routine blood manipulation procedures, specifically centrifugation and hemoconcentration, effectively preserve immature platelets within platelet units.
  • These findings support the continued presence and viability of immature platelets in transfused products.

Keywords:
Absolute immature platelet countCentrifugationHemoconcentrationImmature platelet fractionImmature plateletsPlatelets

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