Related Experiment Video
Updated: Jul 13, 2026
Revealing the Ferroptotic Phenotype of Medulloblastoma
Published on: March 15, 2024
Liposomal nanoparticle-encapsulated polyphenolic vitamins activate ferroptosis in glioma cells by suppressing the
Zhao Gao1, Yuxin Wang2, Shichao Su2
1Department of Neurosurgery, Chinese PLA General Hospital, Beijing 100853, China.
Background:
Vitamin phenol has anti-glioma potential but low bioavailability. Liposomal nanoparticles are effective drug delivery systems. Whether vitamin phenol-encapsulated liposomal nanoparticles can regulate ferroptosis in glioma cells remains unknown.
Objectives:
This study aims to investigate the role of polyphenolic vitamins-encapsulated liposomal nanoparticles in ferroptosis in glioma cells.
Methods:
LNP-Vig nanoparticles were prepared. U87MG cells were divided into control, LNP and LNP-Vig groups, with the latter receiving additional treatments (si-EGFR, pc-DNA EGFR, si-Hes1, or pc-DNA Hes1). Levels of ferroptosis, apoptosis and related proteins (EGFR/Hes1) were then detected.
Results:
The successfully formulated LNP-Vig nanoparticles induced ferroptosis and suppressed EGFR expression in U87MG cells, leading to increased apoptosis upon prolonged incubation. While si-EGFR reduced EGFR mRNA, the pc-DNA EGFR counteracted LNP-Vig's suppression of EGFR. The consistent changes in downstream NOTCH1 and Hes1 mRNA levels confirmed that LNP-Vig influences the NOTCH1/Hes1 pathway via EGFR inhibition. Both NOTCH1 blockade and Hes1 knockdown reduced the levels of ferroptosis-related proteins, whereas NOTCH1 activation or Hes1 overexpression inhibited ferroptosis (P<0.05).
Conclusion:
LNP-Vig promotes apoptosis by activating ferroptosis, an effect crucially dependent on Hes1 suppression. By modulating the EGFR/NOTCH1 pathway, LNP-Vig promotes ferroptosis in U87MG cells, thus identifying a potential new target for cancer treatment.
